Anti-Glycophorin A antibody [JC159], prediluted (Phycoerythrin) (ab197142)

Overview

  • Product name

    Anti-Glycophorin A antibody [JC159], prediluted (Phycoerythrin)
    See all Glycophorin A primary antibodies
  • Description

    Mouse monoclonal [JC159] to Glycophorin A, prediluted (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab197142 does not react with Glycophorin B.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Glycophorin A. (Membrane preparation from splenic hairy cell leukemia).

  • Epitope

    ab197142 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of Glycophorin A.
  • Positive control

    • Human blood cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.0975% Sodium azide
    Constituents: 0.2% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
  • Clonality

    Monoclonal
  • Clone number

    JC159
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

(or 100 µl of whole blood).

 

 

 

 

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similarities

    Belongs to the glycophorin A family.
  • Post-translational
    modifications

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

References

ab197142 has not yet been referenced specifically in any publications.

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