• Product name

    Anti-Glycophorin A antibody [YTH89.1]
    See all Glycophorin A primary antibodies
  • Description

    Rat monoclonal [YTH89.1] to Glycophorin A
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IHC-P: Human hypophysis.



Our Abpromise guarantee covers the use of ab33386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/25.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/1000.

This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

IHC-Fr 1/100 - 1/1000.


  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similarities

    Belongs to the glycophorin A family.
  • Post-translational

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all


  • ab33386 staining erythrocytes in sinusoids of human hypophysis by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
  • ab33386 staining Glycophorin A in isolated human cord blood by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 1% BSA + 0.1% Triton X-100 + 0.05% Tween-20 + 0.01% NaN3. The sample was incubated with the primary antibody (2µg/ml in !% BSA + 0.05% Tween-20 + 0.01% NaN3) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.
    Gating Strategy: Against debris (low SSC/high FSC).

    See Abreview


This product has been referenced in:

  • Jokiranta TS & Meri S Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J Immunol 151:2124-31 (1993). Read more (PubMed: 7688394) »
  • Outram S  et al. Erythromyeloid lineage fidelity is conserved in erythroleukaemia. Leuk Res 12:651-7 (1988). Read more (PubMed: 3184981) »
See all 2 Publications for this product

Customer reviews and Q&As

Flow Cytometry
Yes - 1% BSA, 0.1% Triton X-100, 0.05% Tween-20, 0.01% NaN3
Human Cell (Isolated Human Cord Blood)
Isolated Human Cord Blood
Gating Strategy
Against debris (low SSC/high FSC)
Cell harvesting/tissue preparation method: 4% PFA fixed cells (12h), buffer washed (1% BSA, 0.05% Tween-20, 0.01% NaN3), permeabilised (buffer with 0.1% Triton X100), blocked (10% NGS)
Sample buffer: 1% BSA, 0.05% tween-20, 0.01% NaN3

Mr. Mark Allenby

Verified customer

Submitted Mar 14 2015

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