Overview

  • Product name
    Anti-Glycophorin A antibody [YTH89.1] (FITC)
    See all Glycophorin A primary antibodies
  • Description
    Rat monoclonal [YTH89.1] to Glycophorin A (FITC)
  • Host species
    Rat
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human red blood cells followed by purified glycophorin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab28082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use neat.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
    • Sequence similarities
      Belongs to the glycophorin A family.
    • Post-translational
      modifications
      The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
    • Cellular localization
      Cell membrane. Appears to be colocalized with SLC4A1.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI853584 antibody
      • Blood group--MN locus antibody
      • CD_antigen=CD235a antibody
      • CD235a antibody
      • GLPA_HUMAN antibody
      • Glycophorin A (MNS blood group) antibody
      • Glycophorin A antibody
      • Glycophorin A, included antibody
      • Glycophorin-A antibody
      • GlycophorinA antibody
      • GPA antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPA, included antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS-2 antibody
      • PAS2 antibody
      • Sialoglycoprotein alpha antibody
      see all

    Images

    • Staining of human peripheral blood erythrocytes with neat rat anti human CD235a:FITC (ab28082)

    References

    This product has been referenced in:
    • Nanayakkara NP  et al. Scalable preparation and differential pharmacologic and toxicologic profiles of primaquine enantiomers. Antimicrob Agents Chemother 58:4737-44 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24913163) »
    • Jokiranta TS & Meri S Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J Immunol 151:2124-31 (1993). Read more (PubMed: 7688394) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Gracias por contactarnos.



    Como bien comentas, hay 4 anticuerpos contra Glycophorin A conjugados con FITC. Uno de ellos es policlonal, el ab1112201, y reconoce varios epítopos, mientras que los tres restantes son monoclonales.



    La diferencia principal entre ellos es el proceso de producción y/o purificación. Los inmunógenos usados para generar los anticuerpos varían también. Quizás para tu propósito los más adecuados sean ab51531, o ab28082. Ambos han usado eritrocitos como inmunógeno, y están garantizados para reconocer esas células mediante Citometría de flujo


    Todos nuestros productos están cubiertos por la garantía Abpromise, así que en caso de que el anticuerpo se haya usado siguiendo un protocolo adecuado, y éste no funcionara como se especifica en la ficha técnica, estaremos encantados de ofrecer un reemplazo del producto, un vale por el importe adquirido, o un reembolso.



    También querría comentarte que actualmente tenemos una promoción vigente por la que al pedir cualquiera de nuestros anticuerpos primarios, os regalamos otro primario Rabbit Monoclonal.

    Para leer más sobre esta promoción y conocer cómo proceder con el pedido, te recomiendo leer la siguiente información:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15447



    Espero que esta información sea de utilidad. No dudes en contactarnos de nuevo para cualquier otra consulta.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up