Biotin Anti-Glycophorin A + B antibody [HIR2] (ab93548)
Key features and details
- Biotin Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Biotin Anti-Glycophorin A + B antibody [HIR2]
See all Glycophorin A + B primary antibodies -
Description
Biotin Mouse monoclonal [HIR2] to Glycophorin A + B -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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Positive control
- Human peripheral blood leukocytes
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General notes
Mouse monoclonal [HIR2] to Glycophorin A (Biotin) also has a clone number GA-R2.Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% Gelatin -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Binding of ab93548 to red cells at high antibody concentration causes cell agglutination. -
Clonality
Monoclonal -
Clone number
HIR2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 0.125ug for 10^5 to 10^8 cells in a final volume of 100 ul. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 0.125ug for 10^5 to 10^8 cells in a final volume of 100 ul. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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Alternative names
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
Datasheets and documents
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Datasheet download
References (1)
ab93548 has been referenced in 1 publication.
- Chen G et al. [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria] Zhonghua Xue Ye Xue Za Zhi 18:190-2 (1997). PubMed: 15622765