Recombinant Anti-Glycophorin C/GPC antibody [EPR4115] (ab108619)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Glycophorin C/GPC antibody [EPR4115]
See all Glycophorin C/GPC primary antibodies -
Description
Rabbit monoclonal [EPR4115] to Glycophorin C/GPC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glycophorin C/GPC aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- TF1 and K562 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Glycophorin C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab108619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 14 kDa. | |
| IP | 1/10 - 1/100. | |
| ICC | 1/250 - 1/500. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. -
Tissue specificity
Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous. -
Sequence similarities
Belongs to the glycophorin-C family. -
Cellular localization
Cell membrane. Linked to the membrane via band 4.1. - Information by UniProt
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Database links
- Entrez Gene: 2995 Human
- Omim: 110750 Human
- SwissProt: P04921 Human
- Unigene: 59138 Human
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Alternative names
- CD236 antibody
- CD236R antibody
- GE antibody
see all
Images
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![Western blot - Anti-Glycophorin C/GPC antibody [EPR4115] (ab108619)](https://www.abcam.com/ps/products/108/ab108619/Images/ab108619-121353-anti-glycophorin-c-antibody-epr4115-western-blot.jpg)
Western blot - Anti-Glycophorin C/GPC antibody [EPR4115] (ab108619)All lanes : Anti-Glycophorin C/GPC antibody [EPR4115] (ab108619) at 1/1000 dilution
Lane 1 : TF1 cell lysates
Lane 2 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Maxwell P et al. Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol 168:429-42 (2015). Read more (PubMed: 25283956) »
