Recombinant Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Glycophorin C/GPC antibody [EPR4116]
See all Glycophorin C/GPC primary antibodies -
Description
Rabbit monoclonal [EPR4116] to Glycophorin C/GPC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glycophorin C/GPC. The exact sequence is proprietary.
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Positive control
- TF-1 cell lysate, Red blood cell lysate, K562 cell lysate; human lung tissue, human spleen tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Glycophorin C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 14 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. -
Tissue specificity
Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous. -
Sequence similarities
Belongs to the glycophorin-C family. -
Cellular localization
Cell membrane. Linked to the membrane via band 4.1. - Information by UniProt
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Database links
- Entrez Gene: 2995 Human
- Omim: 110750 Human
- SwissProt: P04921 Human
- Unigene: 59138 Human
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Alternative names
- CD236 antibody
- CD236R antibody
- GE antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121414-anti-glycophorin-c-antibody-epr4116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)Immunohistochemical staining of paraffin-embedded human lung tissue using ab108925 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121413-anti-glycophorin-c-antibody-epr4116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)Immunohistochemical staining of paraffin-embedded human spleen tissue using ab108925 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121412-anti-glycophorin-c-antibody-epr4116-western-blot.jpg)
Western blot - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)All lanes : Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925) at 1/1000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Red blood cell lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121411-anti-glycophorin-c-antibody-epr4116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)ab108925 showing positive staining in Normal ovary erythrocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121410-anti-glycophorin-c-antibody-epr4116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)ab108925 showing positive staining in Normal colon erythrocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/Glycophorin-C-Primary-antibodies-ab108925-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)ab108925 showing positive staining in Normal brain erythrocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)](https://www.abcam.com/ps/products/108/ab108925/Images/ab108925-121408-anti-glycophorin-c-antibody-epr4116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody [EPR4116] (ab108925)ab108925 showing positive staining in Normal tonsil erythrocytes tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
ab108925 has not yet been referenced specifically in any publications.
