Key features and details
- Detection method: Colorimetric
- Sample type: Cell Lysate, Purified protein, Tissue Homogenate
Product nameGlyoxalase I Assay Kit (Colorimetric)
Sample typeCell Lysate, Tissue Homogenate, Purified protein
Glyoxalase I Assay Kit (Colorimetric) (ab241019) utilizes the ability of an active Glyoxalase I (GloI) to catalyze the formation of S-D-lactoylglutathione (SLG) by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm.
Our assay kit is simple, sensitive and can detect as low as 0.05 mU of GloI activity in biological samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests GloI Assay Buffer 1 x 25ml GloI Substrate A 1 x 1.1ml GloI Substrate B (Lyophilized) 1 vial Glyoxalase I (Lyophilized) 1 vial UV transparent plate (96-well) 1 unit
FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similaritiesBelongs to the glyoxalase I family.
- Information by UniProt
Measurement of GloI activity in U937 Cell Lysate (0.6 µg Protein), Hep G2 Cell Lysates (2 µg Protein), Rat Liver tissue extracts (1.5 µg Protein), Rat Kidney tissue extracts (2 µg Protein) & Positive Control.
GloI Activity in U937 Cell Lysates, HepG2 Cell Lysates, Rat Liver tissue extracts and Rat Kidney tissue extracts. All assays were performed following kit protocol.
ab241019 has not yet been referenced specifically in any publications.