Product nameAnti-Glypican 1/ GPC1 antibody
See all Glypican 1/ GPC1 primary antibodies
DescriptionRabbit polyclonal to Glypican 1/ GPC1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rhesus monkey
Recombinant fragment within Human Glypican 1/ GPC1 (internal sequence). The exact sequence is proprietary.
Database link: P35052
- WB: A431 and MCF7 whole cell extracts. IHC-P: Human normal pancreas and pancreatic cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionCell surface proteoglycan that bears heparan sulfate.
Sequence similaritiesBelongs to the glypican family.
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
Lanes 1-4 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution
Lanes 5-6 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract
Lane 3 : A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract
Lane 4 : A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 6 : MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.
ab226855 has not yet been referenced specifically in any publications.