Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody
    See all Glypican 1/ GPC1 primary antibodies
  • Description

    Rabbit polyclonal to Glypican 1/ GPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Glypican 1/ GPC1 (internal sequence). The exact sequence is proprietary.
    Database link: P35052

  • Positive control

    • WB: A431 and MCF7 whole cell extracts. IHC-P: Human normal pancreas and pancreatic cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab226855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • Lanes 1-4 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution
    Lanes 5-6 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract
    Lane 3 : A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract
    Lane 4 : A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract
    Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 6 : MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

References

ab226855 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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