Key features and details
- Rabbit polyclonal to Glypican 1/ GPC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glypican 1/ GPC1 antibody
See all Glypican 1/ GPC1 primary antibodies
DescriptionRabbit polyclonal to Glypican 1/ GPC1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rhesus monkey
Recombinant fragment within Human Glypican 1/ GPC1 (internal sequence). The exact sequence is proprietary.
Database link: P35052
- WB: A431 and MCF7 whole cell extracts. IHC-P: Human normal pancreas and pancreatic cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionCell surface proteoglycan that bears heparan sulfate.
Sequence similaritiesBelongs to the glypican family.
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
Lanes 1-4 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution
Lanes 5-6 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract
Lane 3 : A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract
Lane 4 : A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 6 : MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226855 has not yet been referenced specifically in any publications.