Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody
    See all Glypican 1/ GPC1 primary antibodies
  • Description

    Rabbit polyclonal to Glypican 1/ GPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) corresponding to a C-terminal sequence.

Properties

Applications

Our Abpromise guarantee covers the use of ab55971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent dilution.

Target

Images

  • Anti-Glypican 1/ GPC1 antibody (ab55971) at 1/2000 dilution + lysate prepared from ScN2a cells.

    Secondary
    peroxidase-conjugated secondary

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.

References

This product has been referenced in:

  • Qadir F  et al. Transcriptome reprogramming by cancer exosomes: identification of novel molecular targets in matrix and immune modulation. Mol Cancer 17:97 (2018). Read more (PubMed: 30008265) »
  • Cave C  et al. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord. Mol Neurodegener 12:8 (2017). Read more (PubMed: 28103900) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: ####


As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably recieved a bad vial.

I apologise for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation.

In addition to this, I can suggest you may like to consider trying the secondary antibody with a different primary antibody to ensure the current vial is working if this has not already been tried.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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