Key features and details
- Rabbit polyclonal to Glypican 1/ GPC1
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glypican 1/ GPC1 antibody
See all Glypican 1/ GPC1 primary antibodies
DescriptionRabbit polyclonal to Glypican 1/ GPC1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from the N-terminal domain of human Glypican/GPC1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab73979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/2000. Predicted molecular weight: 62 kDa.|
|IHC-Fr||1/50 - 1/100.|
FunctionCell surface proteoglycan that bears heparan sulfate.
Sequence similaritiesBelongs to the glypican family.
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
ICC/IF image of ab73979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab73979 has been referenced in 1 publication.
- Suhovskih AV et al. Prostate cancer cells specifically reorganize epithelial cell-fibroblast communication through proteoglycan and junction pathways. Cell Adh Migr 11:39-53 (2017). PubMed: 27111714