Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody
    See all Glypican 1/ GPC1 primary antibodies
  • Description

    Rabbit polyclonal to Glypican 1/ GPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N-terminal domain of human Glypican/GPC1

Properties

Applications

Our Abpromise guarantee covers the use of ab73979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 62 kDa.
IHC-Fr 1/50 - 1/100.

Target

Images

  • ICC/IF image of ab73979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Suhovskih AV  et al. Prostate cancer cells specifically reorganize epithelial cell-fibroblast communication through proteoglycan and junction pathways. Cell Adh Migr 11:39-53 (2017). Read more (PubMed: 27111714) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for bringing this to our attention. We have not had other complaints about the antibody, and we ordinarily try to offer suggestions to improve the result, but I assume the protocol you have is correct and that you are not interested in continuing to work with this antibody.

We have other antibodies against this protein, such as ab55971 but I will issue a credit for the cost of ab73979 instead of a replacement, which can be used with your next order of any of our products.

Your credit note ID is 23122.

To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for contacting us.
The immunogen show 80% sequence homology with mouse so it is more likely that the antibody would cross react.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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https://www.abcam.com/abreviews

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Answer

Thank you for your reply.

I am sorry to hearthat the customerhas problemswith this antibody. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. We will replace the antibody if the antibody is not working as we guarantee.

Please provide the protocol information in which the antibody was not performing well. I am attaching our questionnaire so that we can gather this further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Read More

Answer

Thank you for your patience.

I am sorry to let you know that the laboratory has no image to provide, as the antibody has been tested in another lab on Western blot.It has only been tested on Glypican-1 transfected 293T cells lysates as far as I know.

Please let me know if the customer has any questions or concerns. I would be happy to look into it.

Thank you for your cooperation.

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Answer

Thank you for your reply.

I have requested an image from the laboratorzy, however I have not yet received it. I will forward this to you, as soon as we have it. I can however reassure the customer, that we do guarantee this antibody to give good results in Western blot.

In regards to the cells which can be used as positive control, I can recommend HeLa cells for example. As you see also in the IF picture on the datasheet, HeLa cells do contain a good amount of Glypican 1.

I hope this information is helpful. Please do not hesitate to contact us again should you need meanwhile further information.

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Answer

Thank you for your inquiry.

The product ab73979 does not require an MSDS since it doesn't have any preservatives in it. If you need documentation stating this, please let me know.

I hope this informaiton helps. Please contact us with any other questions.

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