Recombinant Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody [EPR19285]
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glypican 1/ GPC1 aa 350-450. The exact sequence is proprietary.
Database link: P35052 -
Positive control
- WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab199343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa). | |
| ICC/IF | 1/500. | |
| Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Sequence similarities
Belongs to the glypican family. -
Post-translational
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2817 Human
- Omim: 600395 Human
- SwissProt: P35052 Human
- Unigene: 328232 Human
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Alternative names
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
see all
Images
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![Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)](https://www.abcam.com/ps/products/199/ab199343/Images/ab199343-266589-anti-glypican-1-gpc1-antibody-epr19285-western-blot.jpg)
Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Additional bands at: 55 kDa (possible post-translational modification)
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the literature (PMID: 9802880).
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![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)](https://www.abcam.com/ps/products/199/ab199343/Images/ab199343-266586-anti-glypican-1-gpc1-antibody-epr19285-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)](https://www.abcam.com/ps/products/199/ab199343/Images/ab199343-266587-anti-glypican-1-gpc1-antibody-epr19285-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)](https://www.abcam.com/ps/products/199/ab199343/Images/ab199343-266585-anti-glypican-1-gpc1-antibody-epr19285-flow-cytometry.jpg)
Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)](https://www.abcam.com/ps/products/199/ab199343/Images/ab199343-266588-anti-glypican-1-gpc1-antibody-epr19285-western-blot.jpg)
Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human colon lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lanes 2 and 3: 10 seconds.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Qiu W et al. Oridonin-loaded and GPC1-targeted gold nanoparticles for multimodal imaging and therapy in pancreatic cancer. Int J Nanomedicine 13:6809-6827 (2018). Read more (PubMed: 30425490) »
- Li G et al. Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer. Glycoconj J N/A:N/A (2017). Read more (PubMed: 28822024) »
