Recombinant
RabMAb

Recombinant Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

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Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody [EPR19285]
    See all Glypican 1/ GPC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Glypican 1/ GPC1 aa 350-450. The exact sequence is proprietary.
    Database link: P35052

  • Positive control

    • WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
ICC/IF 1/500.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate.

  • Sequence similarities

    Belongs to the glypican family.

  • Post-translational
    modifications

    This cell-associated glypican is further processed to give rise to a medium-released species.

  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ38078 antibody
      • glypican antibody
      • Glypican proteoglycan 1 antibody
      • glypican1 antibody
      • GPC 1 antibody
      • Gpc1 antibody
      • GPC1_HUMAN antibody
      • Secreted glypican-1 antibody
      see all

    Images

    • Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

      Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 5 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 61 kDa
      Additional bands at: 55 kDa (possible post-translational modification)


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with the literature (PMID: 9802880).

    • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on MCF7 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

      Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)
      All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human colon lysate
      Lane 3 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 61 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 5 seconds; Lanes 2 and 3: 10 seconds.

    References

    This product has been referenced in:

    • Qiu W  et al. Oridonin-loaded and GPC1-targeted gold nanoparticles for multimodal imaging and therapy in pancreatic cancer. Int J Nanomedicine 13:6809-6827 (2018). Read more (PubMed: 30425490) »
    • Li G  et al. Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer. Glycoconj J N/A:N/A (2017). Read more (PubMed: 28822024) »
    See all 2 Publications for this product

    Publishing research using ab199343? Please let us know so that we can cite the reference in this datasheet.

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