Recombinant Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (ab255600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22580-72] to Glypican 1/ GPC1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody [EPR22580-72]
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit monoclonal [EPR22580-72] to Glypican 1/ GPC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: DU 145 (cultured under serum-free conditions), PC-3, MCF7 and MDA-MB-231 whole cell lysates. IHC-P: Human pancreatic carcinoma, lung carcinoma and esophagus carcinoma tissue. IP: DU145 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22580-72 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa).
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa). |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Sequence similarities
Belongs to the glypican family. -
Post-translational
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2817 Human
- Omim: 600395 Human
- SwissProt: P35052 Human
- Unigene: 328232 Human
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Alternative names
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue (A) and the paired adjacent pancreas tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranous staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID: 28440066) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue (A) and the paired adjacent lung tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human lung carcinoma (panel A) and the paired adjacent lung (panel B) was barely stained (PMID: 29785020) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of paraffin-embedded esophagus lung carcinoma tissue labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on esophagus carcinoma (PMID: 28445969) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab255600 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Glypican 1/ GPC1 was immunoprecipitated from 0.35 mg DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg with ab255600 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255600 1/1000 dilution (0.65 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg
Lane 2: ab255600 IP in DU 145 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255600 in DU 145 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 75 seconds.
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All lanes : Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (ab255600) at 1/1000 dilution
Lane 1 : DU 145 (human prostate carcinoma cell line) cultured under serum-free conditions, whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and dilution biffer: 5% NFDM/TBST.
Molecular weight observed is consistent with what has been described in the literature (PMID: 8207484, 27313791).
Homology with other Glypican family members (38.88% to GPC-2; 21.55% to GPC-3; 43.41% to GPC-4; 25.86% to GPC-5; 45.05% to GPC-6).
Exposure time:
Lanes 1-2: 5.5 seconds
Lanes 3-4: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab255600 has not yet been referenced specifically in any publications.