Overview

  • Product name
    Anti-Glypican 3 antibody [EPR10641]
    See all Glypican 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR10641] to Glypican 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Glypican 3. The exact sequence is proprietary.
    Database link: P51654

  • Positive control
    • HepG2 cell lysate; Human fetal liver lysate; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab174851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
    • Tissue specificity
      Highly expressed in lung, liver and kidney.
    • Involvement in disease
      Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
    • Sequence similarities
      Belongs to the glypican family.
    • Cellular localization
      Cell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab174851 observed at 70 kDa. Red - loading control, ab130007, observed at 125 kDa.

      ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. Ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red)  or a rabbit IgG negative (green).

    • All lanes : Anti-Glypican 3 antibody [EPR10641] (ab174851) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 66 kDa

    References

    ab174851 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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