Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10641] to Glypican 3 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Product nameAnti-Glypican 3 antibody [EPR10641] - BSA and Azide free
See all Glypican 3 primary antibodies
DescriptionRabbit monoclonal [EPR10641] to Glypican 3 - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant fragment. The exact sequence is proprietary.
Database link: P51654
- HepG2 cell lysate; Human fetal liver lysate; HepG2 cells.
Ab221498 is the carrier-free version of ab174851. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab221498 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab221498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionCell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Tissue specificityHighly expressed in lung, liver and kidney.
Involvement in diseaseDefects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
Sequence similaritiesBelongs to the glypican family.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
This data was developed using ab174851, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
ab174851 was shown to recognize Glypican 3 in wild-type HAP1 cells as signal was lost at the expected MW in GPC3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. ab174851 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab221498 has not yet been referenced specifically in any publications.