Product nameAnti-Glypican 3 antibody [EPR5547]
See all Glypican 3 primary antibodies
DescriptionRabbit monoclonal [EPR5547] to Glypican 3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Glypican 3 aa 500-600. The exact sequence is proprietary.
- SH-SY5Y, Jurkat and HepG2 cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
FunctionCell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Tissue specificityHighly expressed in lung, liver and kidney.
Involvement in diseaseDefects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
Sequence similaritiesBelongs to the glypican family.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/50000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysates
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Blocking and diluting buffer:5% NFDM/TBST
Exposure time:15 seconds
This product has been referenced in:
- Cartier F et al. New tumor suppressor microRNAs target glypican-3 in human liver cancer. Oncotarget 8:41211-41226 (2017). WB, Flow Cyt . Read more (PubMed: 28476031) »
- Liu W et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »