Anti-Glypican 3 antibody [EPR5547] (ab124829)


  • Product name
    Anti-Glypican 3 antibody [EPR5547]
    See all Glypican 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5547] to Glypican 3
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Glypican 3 aa 500-600. The exact sequence is proprietary.

  • Positive control
    • SH-SY5Y, Jurkat and HepG2 cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


Associated products


Our Abpromise guarantee covers the use of ab124829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
    • Tissue specificity
      Highly expressed in lung, liver and kidney.
    • Involvement in disease
      Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
    • Sequence similarities
      Belongs to the glypican family.
    • Cellular localization
      Cell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all


    • All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 66 kDa

    • All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/50000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysates
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 66 kDa

      Blocking and diluting buffer:5% NFDM/TBST

      Exposure time:15 seconds


    This product has been referenced in:
    • Cartier F  et al. New tumor suppressor microRNAs target glypican-3 in human liver cancer. Oncotarget 8:41211-41226 (2017). WB, Flow Cyt . Read more (PubMed: 28476031) »
    • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »

    See all 2 Publications for this product

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