Recombinant Anti-Glypican 3 antibody [EPR5547] (ab124829)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5547] to Glypican 3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glypican 3 antibody [EPR5547]
See all Glypican 3 primary antibodies -
Description
Rabbit monoclonal [EPR5547] to Glypican 3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Glypican 3 aa 500-600. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, Jurkat and HepG2 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5547 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa -
All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/50000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysates
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 66 kDaBlocking and diluting buffer:5% NFDM/TBST
Exposure time:15 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124829 has been referenced in 2 publications.
- Cartier F et al. New tumor suppressor microRNAs target glypican-3 in human liver cancer. Oncotarget 8:41211-41226 (2017). WB, Flow Cyt . PubMed: 28476031
- Liu W et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . PubMed: 27313680