Anti-Glypican 3 antibody [GPC3/863] (ab216606)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

Key features and details

  • Mouse monoclonal [GPC3/863] to Glypican 3
  • Suitable for: ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Rat
Human
Application Abreviews Notes
ICC
Use a concentration of 1 - 2 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation was for 30 min at RT.
Notes
ICC
Use a concentration of 1 - 2 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation was for 30 min at RT.

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.

  • Tissue specificity

    Highly expressed in lung, liver and kidney.

  • Involvement in disease

    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

  • Sequence similarities

    Belongs to the glypican family.

  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunocytochemistry analysis of MeOH-fixed HepG2 cells labeling Glypican 3 with ab216606 at 1 ug/ml followed by Goat anti-Mouse (Green). The nuclear counterstain is Reddot (Red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    References (0)

    Publishing research using ab216606? Please let us know so that we can cite the reference in this datasheet.

    ab216606 has not yet been referenced specifically in any publications.

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