Anti-Glypican 3 antibody [GPC3/863] (ab216606)

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Overview

  • Product name

    Anti-Glypican 3 antibody [GPC3/863]
    See all Glypican 3 primary antibodies

  • Description

    Mouse monoclonal [GPC3/863] to Glypican 3

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant full length protein aa 1-580.
    Sequence:

    MAGTVRTACL VVAMLLSLDF PGQAQPPPPP PDATCHQVRS FFQRLQPGLK WVPETPVPGS DLQVCLPKGP TCCSRKMEEK YQLTARLNME QLLQSASMEL KFLIIQNAAV FQEAFEIVVR HAKNYTNAMF KNNYPSLTPQ AFEFVGEFFT DVSLYILGSD INVDDMVNEL FDSLFPVIYT QLMNPGLPDS ALDINECLRG ARRDLKVFGN FPKLIMTQVS KSLQVTRIFL QALNLGIEVI NTTDHLKFSK DCGRMLTRMW YCSYCQGLMM VKPCGGYCNV VMQGCMAGVV EIDKYWREYI LSLEELVNGM YRIYDMENVL LGLFSTIHDS IQYVQKNAGK LTTTIGKLCA HSQQRQYRSA YYPEDLFIDK KVLKVAHVEH EETLSSRRRE LIQKLKSFIS FYSALPGYIC SHSPVAENDT LCWNGQELVE RYSQKAARNG MKNQFNLHEL KMKGPEPVVS QIIDKLKHIN QLLRTMSMPK GRVLDKNLDE EGFESGDCGD DEDECIGGSG DGMIKVKNQL RFLAELAYDL DVDDAPGNSQ QATPKDNEIS TFHNLGNVHS PLKLLTSMAI SVVCFFFLVH


    Database link: P51654

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human hepatocellular carcinoma, rat lung, rat heart and human melanoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab216606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation was for 30 min at RT.

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.

  • Tissue specificity

    Highly expressed in lung, liver and kidney.

  • Involvement in disease

    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

  • Sequence similarities

    Belongs to the glypican family.

  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    References

    ab216606 has not yet been referenced specifically in any publications.

    Publishing research using ab216606? Please let us know so that we can cite the reference in this datasheet.

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