Anti-Glypican 3 antibody [GPC3/863] (ab216606)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)](https://www.abcam.com/ps/products/216/ab216606/Images/ab216606-270196-anti-glypican-3-antibody-gpc3-863-immunohistochemistry-hepatocellular-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)")
Key features and details
- Mouse monoclonal [GPC3/863] to Glypican 3
- Suitable for: ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Glypican 3 antibody [GPC3/863]
See all Glypican 3 primary antibodies -
Description
Mouse monoclonal [GPC3/863] to Glypican 3 -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein aa 1 to the C-terminus.
Database link: P51654 -
Positive control
- IHC-P: Human hepatocellular carcinoma and melanoma tissues; Rat lung and heart tissues. ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab216606 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
GPC3/863 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 1 - 2 µg/ml.
|
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| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation was for 30 min at RT. |
| Notes |
|---|
|
ICC
Use a concentration of 1 - 2 µg/ml. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation was for 30 min at RT. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Entrez Gene: 25236 Rat
- Omim: 300037 Human
- SwissProt: P51654 Human
- SwissProt: P13265 Rat
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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![Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)](https://www.abcam.com/ps/products/216/ab216606/Images/ab216606-453196-anti-glypican-3-antibody-gpc3-863-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)Immunocytochemistry analysis of MeOH-fixed HepG2 cells labeling Glypican 3 with ab216606 at 1 ug/ml followed by Goat anti-Mouse (Green). The nuclear counterstain is Reddot (Red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)](https://www.abcam.com/ps/products/216/ab216606/Images/ab216606-270194-anti-glypican-3-antibody-gpc3-863-immunohistochemistry-lung-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)](https://www.abcam.com/ps/products/216/ab216606/Images/ab216606-270193-anti-glypican-3-antibody-gpc3-863-immunohistochemistry-heart-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)](https://www.abcam.com/ps/products/216/ab216606/Images/ab216606-270192-anti-glypican-3-antibody-gpc3-863-immunohistochemistry-melanoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
Datasheets and documents
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Datasheet download
References (0)
ab216606 has not yet been referenced specifically in any publications.
