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Stem Cells Lineage Markers Mesoderm
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Anti-Glypican 3 antibody [GPC3/863] (ab216606)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

Key features and details

  • Mouse monoclonal [GPC3/863] to Glypican 3
  • Suitable for: ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Glypican 3 antibody [GPC3/863]
    See all Glypican 3 primary antibodies
  • Description

    Mouse monoclonal [GPC3/863] to Glypican 3
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus.
    Database link: P51654

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human hepatocellular carcinoma and melanoma tissues; Rat lung and heart tissues. ICC: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab216606 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    GPC3/863
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Signal Transduction
    • Cytoskeleton / ECM
    • HSPGs
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Associated products

  • Alternative Versions

    • Anti-Glypican 3 antibody [GPC3/863] - BSA and Azide free (ab212411)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Rat
Human
Application Abreviews Notes
ICC
Use a concentration of 1 - 2 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation was for 30 min at RT.

Notes
ICC
Use a concentration of 1 - 2 µg/ml.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation was for 30 min at RT.

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
  • Tissue specificity

    Highly expressed in lung, liver and kidney.
  • Involvement in disease

    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
  • Sequence similarities

    Belongs to the glypican family.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Target information above from: UniProt accession P51654 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2719 Human
    • Entrez Gene: 25236 Rat
    • Omim: 300037 Human
    • SwissProt: P51654 Human
    • SwissProt: P13265 Rat
    • Unigene: 644108 Human
    • Unigene: 9717 Rat
    • Alternative names

      • DGSX antibody
      • Glypican proteoglycan 3 antibody
      • Glypican-3 [Precursor] antibody
      • Gpc3 antibody
      • GPC3_HUMAN antibody
      • GTR2 2 antibody
      • GTR2-2 antibody
      • Heparan sulphate proteoglycan antibody
      • Intestinal protein OCI 5 antibody
      • Intestinal protein OCI-5 antibody
      • MXR7 antibody
      • OCI 5 antibody
      • OCI-5 antibody
      • OCI5 antibody
      • SDYS antibody
      • Secreted glypican-3 antibody
      • SGB antibody
      • SGBS antibody
      • SGBS1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunocytochemistry - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunocytochemistry analysis of MeOH-fixed HepG2 cells labeling Glypican 3 with ab216606 at 1 ug/ml followed by Goat anti-Mouse (Green). The nuclear counterstain is Reddot (Red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab216606? Please let us know so that we can cite the reference in this datasheet.

    ab216606 has not yet been referenced specifically in any publications.

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