Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Key features and details
- Mouse monoclonal [GPC3/863] to Glypican 3
- Suitable for: ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Glypican 3 antibody [GPC3/863]
See all Glypican 3 primary antibodies -
Description
Mouse monoclonal [GPC3/863] to Glypican 3 -
Host species
Mouse -
Tested Applications & Species
Application Species ICC HumanIHC-P RatHuman -
Immunogen
Recombinant full length protein aa 1 to the C-terminus.
Database link: P51654 -
Positive control
- IHC-P: Human hepatocellular carcinoma and melanoma tissues; Rat lung and heart tissues. ICC: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab216606 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
GPC3/863 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Human
|
IHC-P |
Rat
Human
|
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 1 - 2 µg/ml.
|
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation was for 30 min at RT. |
Notes |
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ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation was for 30 min at RT. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Entrez Gene: 25236 Rat
- Omim: 300037 Human
- SwissProt: P51654 Human
- SwissProt: P13265 Rat
- Unigene: 644108 Human
- Unigene: 9717 Rat
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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Immunocytochemistry analysis of MeOH-fixed HepG2 cells labeling Glypican 3 with ab216606 at 1 ug/ml followed by Goat anti-Mouse (Green). The nuclear counterstain is Reddot (Red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [GPC3/863] (ab216606)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Glypican 3 with ab216606 at 1 μg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216606 has not yet been referenced specifically in any publications.