Anti-Glypican 3 antibody [GPC3/863] - BSA and Azide free (ab212411)
Key features and details
- Mouse monoclonal [GPC3/863] to Glypican 3 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Glypican 3 antibody [GPC3/863] - BSA and Azide free
See all Glypican 3 primary antibodies -
Description
Mouse monoclonal [GPC3/863] to Glypican 3 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Glypican 3 aa 1-580.
Database link: P51654 -
Positive control
- 293T cells or Hepatocellular carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G -
Clonality
Monoclonal -
Clone number
GPC3/863 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Entrez Gene: 25236 Rat
- Omim: 300037 Human
- SwissProt: P51654 Human
- SwissProt: P13265 Rat
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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Immunohistochemical analysis of formalin fixed paraffin embedded Human Melanoma labeling Glypican 3 using ab212411 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin fixed paraffin embedded Rat heart tissue labeling Glypican 3 using ab212411 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin fixed paraffin embedded Rat lung tissue labeling Glypican 3 using ab212411 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin fixed paraffin embedded Human hepatocellular carcinoma tissue labeling Glypican 3 using ab212411 at 1 μg/ml dilution.
Datasheets and documents
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Datasheet download
References (0)
ab212411 has not yet been referenced specifically in any publications.