Recombinant

Recombinant Anti-Glypican 3 antibody [rGPC3/863] (ab219313)

Overview

  • Product name

    Anti-Glypican 3 antibody [rGPC3/863]
    See all Glypican 3 primary antibodies
  • Description

    Mouse monoclonal [rGPC3/863] to Glypican 3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human Glypican 3.
    Database link: P51654

  • Positive control

    • Human hepatocellular carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab219313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array Use at an assay dependent concentration.

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
  • Tissue specificity

    Highly expressed in lung, liver and kidney.
  • Involvement in disease

    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
  • Sequence similarities

    Belongs to the glypican family.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • DGSX antibody
    • Glypican proteoglycan 3 antibody
    • Glypican-3 [Precursor] antibody
    • Gpc3 antibody
    • GPC3_HUMAN antibody
    • GTR2 2 antibody
    • GTR2-2 antibody
    • Heparan sulphate proteoglycan antibody
    • Intestinal protein OCI 5 antibody
    • Intestinal protein OCI-5 antibody
    • MXR7 antibody
    • OCI 5 antibody
    • OCI-5 antibody
    • OCI5 antibody
    • SDYS antibody
    • Secreted glypican-3 antibody
    • SGB antibody
    • SGBS antibody
    • SGBS1 antibody
    see all

Images

  • ab219313 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded human hepatocellular carcinoma tissue labeling Glypican 3 with ab219313 at 1 μg/ml.

References

ab219313 has not yet been referenced specifically in any publications.

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