Anti-Glypican 3 antibody [SP86] (ab95363)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Glypican 3 antibody [SP86]
See all Glypican 3 primary antibodies -
DescriptionRabbit monoclonal [SP86] to Glypican 3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Glypican 3 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P51654 -
Positive control
- Human liver cancer tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityProtein A/G purified
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Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP86
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab95363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 66 kDa. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval (boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min). |
Target
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FunctionCell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
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Tissue specificityHighly expressed in lung, liver and kidney.
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Involvement in diseaseDefects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
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Sequence similaritiesBelongs to the glypican family.
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Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
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Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
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Alternative names
- DGSX antibody
- Glypican proteoglycan 3 antibody
- Glypican-3 [Precursor] antibody
see all
Images
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![Western blot - Anti-Glypican 3 antibody [SP86] (ab95363)](https://www.abcam.com/ps/products/95/ab95363/Images/ab95363-299028-ab95363KOWBJRIZ.jpg)
Western blot - Anti-Glypican 3 antibody [SP86] (ab95363)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GPC3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab95363 observed at 70 kDa. Red - loading control, ab130007, observed at 125kDa.
ab95363 was shown to specifically react with Glypican 3 in wild-type HAP1 cells as signal was lost in GPC3 knockout cells. Wild-type and GPC3 knockout samples were subjected to SDS-PAGE. ab95363 and ab130007 (Mouse anti-vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)](https://www.abcam.com/ps/products/95/ab95363/Images/ab95363-249534-95363IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)Immunohistochemical staining of human liver hepatocellular carcinoma with ab95363.
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![Flow Cytometry - Anti-Glypican 3 antibody [SP86] (ab95363)](https://www.abcam.com/ps/products/95/ab95363/Images/ab95363-215285-ab95363-Flow.jpg)
Flow Cytometry - Anti-Glypican 3 antibody [SP86] (ab95363)Flow cytometric analysis of rabbit anti-Glypican 3 (SP86) antibody ab98363 (1/100) in HEPG2 cellls (green) compared to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)](https://www.abcam.com/ps/products/95/ab95363/Images/Glypican-3-Primary-antibodies-ab95363-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 3 antibody [SP86] (ab95363)ab95363, at 1/100 dilution, staining Glypican 3 in formalin-fixed, paraffin-embedded Human liver cancer tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [SP86] (ab95363)](https://www.abcam.com/ps/products/95/ab95363/Images/Glypican-3-Primary-antibodies-ab95363-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [SP86] (ab95363)ICC/IF image of ab95363 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
ab95363 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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