Overview

  • Product name

  • Description

    Rabbit polyclonal to Glypican 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human Glypican 4 aa 473-533.
    Sequence:

    SKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKS ANEKADSAGVR


    Database link: O75487

  • Positive control

    • IHC-P: Human pancreas, liver and kidney tissue. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab246973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

Images

  • PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for Glypican 4 (green) using ab246973 at 4μg/ml in ICC/IF.

  • Paraffin-embedded human kidney tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.
  • Paraffin-embedded human liver tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.
  • Paraffin-embedded human pancreas tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.
  • Paraffin-embedded human tonsil tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis. No reactivity in geminal center cells, as expected.

References

ab246973 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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