Western blot abreview for Anti-Glypican 4 antibody [EPR11271]

Excellent
Abreviews
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk (blocking buffer)
Dilution
1/1000

Secondary antibody

Name
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 55 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
Mouse embryonic stem cells in lanes 1 and 2.

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Submitted Nov 28 2013

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