Western blot abreview for Anti-Glypican 4 antibody [EPR11271]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (hESC, cancer-various)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hESC, cancer-various
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Positive control
HUES7, MCF7, MET5A human cell lines.

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Submitted Aug 18 2015

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