Western blot abreview for Anti-Glypican 4 antibody [EPR11271]

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Dako goat anti rabbit IgG HRP P0448
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50 kDa

Additional data

Additional Notes
Very faint band seen at 50kDa, slightly lower than expected.
Abcam response
Clearly not a successful WB, unfortunately. Nevertheless, the antibody is supported to be used in WB of human samples.

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Submitted Nov 13 2015

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