Western blot abreview for Anti-Glypican 4 antibody [EPR11271]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
HUES7 ES cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Dako goat anti rabbit IgG HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

Additional data

Additional Notes
Single specific 55kDa band, but not at suggested MW of 62kDa. Possibly detecting Glypican 4 isoform 2 in human ES cells.

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Submitted Feb 05 2016

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