RecombinantRabMAb

Recombinant Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

Submit a review Submit a question References (3)
Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
  • Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6756(B)] to Glypican 5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glypican 5 antibody [EPR6756(B)]
    See all Glypican 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR6756(B)] to Glypican 5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Glypican 5 aa 1-100 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • A549, Human fetal lung, HepG2 and C6 lysates; Human brain tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.98 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6756(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    Images

    • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (Unpurified)

      Lane 1 : A549 cell lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 63 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

      Unpurified ab124886, at 1/250 dilution, staining Glypican 5 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Immunohistochemical staining of paraffin embedded human glioma with purified ab124886 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified)

      Lane 1 : C6 whole cell lysate
      Lane 2 : Mouse spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + Human fetal lung at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Western blot - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + A549 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      OI-RD Scanning - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
      Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)

    References (3)

    Publishing research using ab124886? Please let us know so that we can cite the reference in this datasheet.

    ab124886 has been referenced in 3 publications.

    • Takeuchi M  et al. Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation. PLoS One 10:e0126562 (2015). WB ; Human . PubMed: 25978455
    • Dhir A  et al. Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs. Nat Struct Mol Biol 22:319-27 (2015). WB ; Human . PubMed: 25730776
    • Li Y  et al. The overexpression of glypican-5 promotes cancer cell migration and is associated with shorter overall survival in non-small cell lung cancer. Oncol Lett 6:1565-1572 (2013). WB ; Human . PubMed: 24260047

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