Recombinant Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6756(B)] to Glypican 5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Glypican 5 antibody [EPR6756(B)]
See all Glypican 5 primary antibodies -
Description
Rabbit monoclonal [EPR6756(B)] to Glypican 5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Glypican 5 aa 1-100 (internal sequence). The exact sequence is proprietary.
-
Positive control
- A549, Human fetal lung, HepG2 and C6 lysates; Human brain tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.98 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6756(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab124886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa). | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Cell surface proteoglycan that bears heparan sulfate. -
Tissue specificity
In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
Database links
- Entrez Gene: 2262 Human
- Entrez Gene: 103978 Mouse
- Entrez Gene: 306157 Rat
- Omim: 602446 Human
- SwissProt: P78333 Human
- SwissProt: Q8CAL5 Mouse
- Unigene: 655675 Human
- Unigene: 320791 Mouse
see all -
Alternative names
- bA93M14.1 antibody
- glypican 5 antibody
- Glypican 5 precursor antibody
see all
Images
-
All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (Unpurified)
Lane 1 : A549 cell lysate
Lane 2 : Human fetal lung lysate
Lane 3 : HepG2 cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)
Unpurified ab124886, at 1/250 dilution, staining Glypican 5 in paraffin-embedded Human brain tissue by Immunohistochemistry.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 5 antibody [EPR6756(B)] (ab124886)Immunohistochemical staining of paraffin embedded human glioma with purified ab124886 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
-
All lanes : Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified)
Lane 1 : C6 whole cell lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + Human fetal lung at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-Glypican 5 antibody [EPR6756(B)] (ab124886) at 1/1000 dilution (purified) + A549 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Protocols
References (3)
ab124886 has been referenced in 3 publications.
- Takeuchi M et al. Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation. PLoS One 10:e0126562 (2015). WB ; Human . PubMed: 25978455
- Dhir A et al. Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs. Nat Struct Mol Biol 22:319-27 (2015). WB ; Human . PubMed: 25730776
- Li Y et al. The overexpression of glypican-5 promotes cancer cell migration and is associated with shorter overall survival in non-small cell lung cancer. Oncol Lett 6:1565-1572 (2013). WB ; Human . PubMed: 24260047