Product nameAnti-GLYR1 antibody [EPR10076(B)]
See all GLYR1 primary antibodies
DescriptionRabbit monoclonal [EPR10076(B)] to GLYR1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human GLYR1 (Uniprot: Q49A26)
- Jurkat, HeLa, K562 and HUVEC cell lysates; Human colon and skin tissues.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).
Sequence similaritiesBelongs to the 3-hydroxyisobutyrate dehydrogenase family. NP60 subfamily.
Contains 1 A.T hook DNA-binding domain.
Contains 1 PWWP domain.
DomainThe A.T hook DNA-binding domain is required for the interaction with MAPK14.
The PWWP domain probably mediates the binding to H3K36me3.
- Information by UniProt
- 3 hydroxyisobutyrate dehydrogenase like protein antibody
- 3-hydroxyisobutyrate dehydrogenase-like protein antibody
- BM045 antibody
All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.
ab154838 has not yet been referenced specifically in any publications.