Overview

  • Product name
    Anti-GM-CSF antibody [30-4]
    See all GM-CSF primary antibodies
  • Description
    Mouse monoclonal [30-4] to GM-CSF
  • Host species
    Mouse
  • Specificity
    When immobilised on a microplate, this antibody will capture recombinant and natural human GM-CSF.
  • Tested applications
    Suitable for: WB, ELISA, Functional Studies, ICC/IFmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab54429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
ELISA 1/1000 - 1/2000.
Functional Studies Use at an assay dependent dilution.
ICC/IF Use a concentration of 25 µg/ml.
  • Application notes
    Is unsuitable for IHC-Fr.
  • Target

    • Function
      Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similarities
      Belongs to the GM-CSF family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • MGI1GM antibody
      • Molgramostin antibody
      • Pluripoietin-a antibody
      • Sargramostim antibody
      see all

    Images

    • PBMCs fixed with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media and adjust to approximately 1 x 106 cells/mL. Cells pelleted, resuspended and 5 ul (5 x 103) cells added to a gelatin coated slide. Liquid evaporated over a low heat and stained using ab54429 at 10 ug/ml.

    References

    This product has been referenced in:
    • Pasquier J  et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). Read more (PubMed: 29180664) »
    • Isik G  et al. Chimeric HIV-1 envelope glycoproteins with potent intrinsic granulocyte-macrophage colony-stimulating factor (GM-CSF) activity. PLoS One 8:e60126 (2013). Human . Read more (PubMed: 23565193) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil

    Mr. Ali Hosseini

    Verified customer

    Submitted Oct 27 2014

    Answer

    Comme convenu, J'ai mis en place l'envoi de :

    une unité d'anti-GM CSFab77768 (https://www.abcam.com/ab77768) en remplacement du ab54429 commandé le 15 février 2012, le numéro de commande de ce produit de remplacement gratuit est : ******.

    une unité d'anti-Calreticulin ab14234 (https://www.abcam.com/ab14234) en remplacement du ab54429 commandé le 4 juillet 2011. le numéro de commande de ce produit de remplacement gratuit est :*******.



    Vous recevrez prochainementles emails de confirmation comprenant les détails d'expédition.

    N'hésitez pas à nous contacter lors d'une prochaine occasion et encore toutes nos excuses pour le désagrément occasionné par l'anticorps ab54429.

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    Answer

    Merci de nous avoir contactés.

    Le laboratoire m'a envoyé l'image obtenue avec le ab54429 (10µg/ml) en ICC sur cellules mononucléées du sang périphérique (PBMC) fixées en PFA.

    Le protocole utilisé est le suivant :

    1. Fix the cells with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media and adjust to approximately 1 x 106 cells/mL.

    2. Add 1 mL of the cell solution to a 1.5 mL microfuge tube.

    3. Spin down the cells for 30 seconds in a microfuge.

    4. Pour off the supernatant and resuspend the cell pellet in 1 mL of deionized H2O.

    5. Spin down the cells for 30 seconds in the microfuge.

    6. Pour off the supernatant, and resuspend the cell pellet in 200 µL of deionized H2O.

    7. Add 5 µL of the cell suspension to a gelatin-coated slide (3 spots per slide), and smear with the side of a pipette tip.

    8. Place the slide on a hot plate (low heat setting), and allow the liquid to evaporate.

    9. Check the slide under a microscope, and make sure that there are no salt crystals. If salt crystals are observed, wash the slide with water.

    10. Surround the cell spot with a hydrophobic barrier using a barrier pen and air dry.

    11. The slides can be stored at 2-8 °C for up to 3 months or they may be stained immediately



    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous recontrez toujours des problèmes. Nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (si le problème nous est communiqué dans les 6 mois qui suivent la commande), nous pouvons donc toujours résoudre le problème que vous rencontrez en vous remboursant, vous faire un avoir, ou vous envoyer un anticorps de remplacement.

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    Question
    Answer

    Thank you very much for your interest in ab54429. To our knowledge, ab54429 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab54429 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab54429 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab54429 so please wait for my reply before ordering. 2. Purchase ab54429 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab54429 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Answer

    According to our records, ab54429, ab25123, ab1420 and ab8098 was proving difficult to use and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to a reply.  

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    Answer

    Thank you for contacting us. This antibody has been raised against recombinant human GM-CSF produced in E. coli. I hoe this information has been helpful. If you have any further questions, please do not hesitate to ask.

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    Answer

    Thank you for confirming the details. I would need the purchase order number to send you the requested antibodies. I am sorry without correct purchase order number of each antibody; I will not be able to send you these products. I hope this will help. I will look forward to hearing from you soon.

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    Question
    Answer

    Thank you for your enquiry regarding ab1420, ab8098, ab54429 and ab25123 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has been very beneficial in understanding your concerns. The protocol looks fine to me however I also would like to ask few questions which would help me identify the source of the problem; - Have you used same lysates with all these antibodies? - You have also ordered many different antibodies; could you confirm that those antibodies worked well using the same protocol? - Does any of these products affected by any shipping delays? - Could you confirm the order number of these products? I will look forward to hearing from you soon.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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