• Product name
    Anti-GM-CSF antibody [30-4]
    See all GM-CSF primary antibodies
  • Description
    Mouse monoclonal [30-4] to GM-CSF
  • Host species
  • Specificity
    When immobilised on a microplate, this antibody will capture recombinant and natural human GM-CSF.
  • Tested applications
    Suitable for: WB, ELISA, Functional Studies, ICC/IFmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF).

  • Positive control



Our Abpromise guarantee covers the use of ab54429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
ELISA 1/1000 - 1/2000.
Functional Studies Use at an assay dependent dilution.
ICC/IF Use a concentration of 25 µg/ml.
  • Application notes
    Is unsuitable for IHC-Fr.
  • Target

    • Function
      Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    • Sequence similarities
      Belongs to the GM-CSF family.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor 2 (granulocyte-macrophage) antibody
      • Colony Stimulating Factor 2 antibody
      • Colony stimulating factor antibody
      • Colony-stimulating factor antibody
      • CSF 2 antibody
      • CSF antibody
      • CSF2 antibody
      • CSF2_HUMAN antibody
      • GM-CSF antibody
      • GMCSF antibody
      • Granulocyte Macrophage Colony Stimulating Factor antibody
      • Granulocyte-macrophage colony-stimulating factor antibody
      • MGC131935 antibody
      • MGC138897 antibody
      • MGI1GM antibody
      • Molgramostin antibody
      • Pluripoietin-a antibody
      • Sargramostim antibody
      see all


    • PBMCs fixed with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media and adjust to approximately 1 x 106 cells/mL. Cells pelleted, resuspended and 5 ul (5 x 103) cells added to a gelatin coated slide. Liquid evaporated over a low heat and stained using ab54429 at 10 ug/ml.


    This product has been referenced in:
    • Pasquier J  et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). Read more (PubMed: 29180664) »
    • Isik G  et al. Chimeric HIV-1 envelope glycoproteins with potent intrinsic granulocyte-macrophage colony-stimulating factor (GM-CSF) activity. PLoS One 8:e60126 (2013). Human . Read more (PubMed: 23565193) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Immunohistochemistry (Resin sections)
    Human Tissue sections (Tonsil)

    Mr. Ali Hosseini

    Verified customer

    Submitted Oct 27 2014

    Comme convenu, J'ai mis en place l'envoi de :

    une unité d'anti-GM CSFab77768 (https://www.abcam.com/ab77768) en remplacement du ab54429 commandé le 15 février 2012, le numéro de commande de ce produit de remplaceme...

    Read More

    Merci de nous avoir contactés.

    Le laboratoire m'a envoyé l'image obtenue avec le ab54429 (10µg/ml) en ICC sur cellules mononucléées du sang périphérique (PBMC) fixées en PFA.


    Read More

    Thank you very much for your interest in ab54429. To our knowledge, ab54429 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab54429 now, test it in IHC-P and submit feedback to us in the form of an Abrev...

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    According to our records, ab54429, ab25123, ab1420 and ab8098 was proving difficult to use and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us be...

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    Thank you for contacting us. This antibody has been raised against recombinant human GM-CSF produced in E. coli. I hoe this information has been helpful. If you have any further questions, please do not hesitate to ask.

    Thank you for confirming the details. I would need the purchase order number to send you the requested antibodies. I am sorry without correct purchase order number of each antibody; I will not be able to send you these products. I hope th...

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    Thank you for your enquiry regarding ab1420, ab8098, ab54429 and ab25123 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for sup...

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