Overview

  • Product name
  • Description
    Rabbit polyclonal to GM-CSF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Highly pure (>98%) recombinant mGM-CSF (mouse Granulocyte Macrophage Colony Stimulating Factor)

  • Positive control
    • Recombinant mouse GM-CSF protein (ab9742) can be used as a positive control in WB. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab9741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect mGM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.6 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similarities
    Belongs to the GM-CSF family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Colony stimulating factor 2 (granulocyte-macrophage) antibody
    • Colony Stimulating Factor 2 antibody
    • Colony stimulating factor antibody
    • Colony-stimulating factor antibody
    • CSF 2 antibody
    • CSF antibody
    • CSF2 antibody
    • CSF2_HUMAN antibody
    • GM-CSF antibody
    • GMCSF antibody
    • Granulocyte Macrophage Colony Stimulating Factor antibody
    • Granulocyte-macrophage colony-stimulating factor antibody
    • MGC131935 antibody
    • MGC138897 antibody
    • MGI1GM antibody
    • Molgramostin antibody
    • Pluripoietin-a antibody
    • Sargramostim antibody
    see all

Images

  • Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

  • Western blot analysis of recombinant GM-CSF using ab9741 at 0.1μg/ml. Bottom band - dye front.

  • Less than 0.10 ug/ml ab9741 will neutralize 50% of the bioactivity of 1 ng/ml m-GM-CSF.
  • IHC image of GM-CSF staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9741, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Liu H  et al. Decreased expression of granulocyte-macrophage colony-stimulating factor is associated with adverse clinical outcome in patients with gastric cancer undergoing gastrectomy. Oncol Lett 14:4701-4707 (2017). Read more (PubMed: 28943964) »
  • Bayih AG  et al. Immune Response and Protective Efficacy of a Heterologous DNA-Protein Immunization withLeishmaniaSuperoxide Dismutase B1. Biomed Res Int 2017:2145386 (2017). WB ; Mouse . Read more (PubMed: 29359145) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us and sorry for the delay in getting back to you.

The neutralizing and inhibition functions stated on the datasheet of the antibodies ab9741 andab64715 relate to the assays which have been performed to validate their activities.

With the Anti-GM-CSF antibody (ab9741), the antibody has been used in a neutralising assay as described in the protocol attached to this email.

Whilst with the Anti-TGF beta 1 antibody [2Ar2] (ab64715) has been used in an inhibition of proliferation assay. This was carried out asdescribed in Tsang, M. et al., (1995) Cytokine 7:389.The dose response curves are given in figures 1 and 2 of the attached document.The response is linear over a small range.

I hope this information has been of help. If you require any further information, please do not hesitate to ask.

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Answer

Thank you very much for your inquiry.

The immunogen of ab9741 was the whole GM-CSFprotein of mouse. Mouse GM-CSF only shows 71% homology with rat as a whole protein and therefore I cannot recommend to use this antibody for rat samples.

We do have two antibodies that have a immunogen (peptide) that is taken from a part of the GM-CSF human or ratsequence that shows a higher homology to the rat sequence. Both antibodies are predicted to react with rat and I would be happy to issue a discount code for either of them.

Maybe your customer would like to review the datasheets and choose one of them for testing in rat?

ab77768

https://www.abcam.com/index.html?datasheet=77768 (or use the following: https://www.abcam.com/index.html?datasheet=77768).

ab114987

https://www.abcam.com/index.html?datasheet=114987 (or use the following: https://www.abcam.com/index.html?datasheet=114987).

Please let me know if your customer would like to take this offer and I will be happy to issue a testing discount code for them.

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Answer

Thank you for contacting us with your question.
The GM-CSF protein is expected to run around 16 kDa, however there are 4 known glycosylation sites, each adding around 2 kDa to the protein, so the protein generally runs around 24 kDa on a Western blot.
I hope this information will be useful, but if you have any further questions or if there is anything else we can do for you, please let me know and I'll be happy to help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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