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    gm130-antibody-ab32337.pdf

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Anti-GM130 antibody (ab32337)

  • Datasheet
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Western blot - Anti-GM130 antibody (ab32337)

    Key features and details

    • Rabbit polyclonal to GM130
    • Suitable for: WB
    • Reacts with: Drosophila melanogaster
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human GM130 protein (ab131818)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GM130 antibody
      See all GM130 primary antibodies
    • Description

      Rabbit polyclonal to GM130
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Drosophila melanogaster
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to GM130 aa 1-100 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab32336)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Golgi
      • Signal Transduction
      • Protein Trafficking
      • Golgi Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human GM130 protein (ab131818)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab32337 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Drosophila melanogaster
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).

    Target

    • Function

      Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
    • Sequence similarities

      Belongs to the GOLGA2 family.
    • Domain

      Extended rod-like protein with coiled-coil domains.
    • Cellular localization

      Golgi apparatus > Golgi stack membrane.
    • Target information above from: UniProt accession Q08379 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • Gm130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • Golga2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Images

    • Western blot - Anti-GM130 antibody (ab32337)
      Western blot - Anti-GM130 antibody (ab32337)
      Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab32337? Please let us know so that we can cite the reference in this datasheet.

    ab32337 has been referenced in 3 publications.

    • Wang J  et al. Exosome-Mediated Transfer of lncRNA HOTTIP Promotes Cisplatin Resistance in Gastric Cancer Cells by Regulating HMGA1/miR-218 Axis. Onco Targets Ther 12:11325-11338 (2019). PubMed: 31908497
    • Maier PJ  et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014). ICC/IF . PubMed: 24668805
    • O'Sullivan NC  et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Drosophila melanogaster . PubMed: 22543973

    Customer reviews and Q&As

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