Anti-GM130 antibody (ab32337)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-GM130 antibody
See all GM130 primary antibodies -
DescriptionRabbit polyclonal to GM130
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Drosophila melanogaster
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.
Read Abcam's proprietary immunogen policy (Peptide available as ab32336.) -
Positive control
- Drosophila S2 Lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 22543973 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). |
Target
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FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
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Sequence similaritiesBelongs to the GOLGA2 family.
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DomainExtended rod-like protein with coiled-coil domains.
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Cellular localizationGolgi apparatus > Golgi stack membrane.
- Information by UniProt
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Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Images
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Western blot - Anti-GM130 antibody (ab32337)Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References
This product has been referenced in:
- Maier PJ et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014). ICC/IF . Read more (PubMed: 24668805) »
- O'Sullivan NC et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22543973) »
