Overview

  • Product name
    Anti-GM130 antibody
    See all GM130 primary antibodies
  • Description
    Rabbit polyclonal to GM130
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) GM130.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab32336.)

  • Positive control
    • Drosophila S2 Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22543973
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).

Target

  • Function
    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • Sequence similarities
    Belongs to the GOLGA2 family.
  • Domain
    Extended rod-like protein with coiled-coil domains.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Alternative names
    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all

Images

  • Western blot - Anti-GM130 antibody (ab32337)
    Western blot - Anti-GM130 antibody (ab32337)
    Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Maier PJ  et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014). ICC/IF . Read more (PubMed: 24668805) »
  • O'Sullivan NC  et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22543973) »

See all 2 Publications for this product

Publishing research using ab32337? Please let us know so that we can cite the reference in this datasheet.

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