Anti-GM130 antibody - BSA and Azide free (ab169276)
Key features and details
- Mouse polyclonal to GM130 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GM130 antibody - BSA and Azide free
See all GM130 primary antibodies -
Description
Mouse polyclonal to GM130 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein, corresponding to amino acids 1-990 of Human GM130 (NP_004477.2).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
|
| ICC/IF | (4) |
Use a concentration of 7 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
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ICC/IF
Use a concentration of 7 µg/ml. |
Target
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Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
Sequence similarities
Belongs to the GOLGA2 family. -
Domain
Extended rod-like protein with coiled-coil domains. -
Cellular localization
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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Database links
- Entrez Gene: 2801 Human
- Omim: 602580 Human
- SwissProt: Q08379 Human
- Unigene: 155827 Human
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Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - BSA and Azide free (ab169276)
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for GM130 (green) using ab169276 at 7 µg/ml in ICC/IF.
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Western blot - Anti-GM130 antibody - BSA and Azide free (ab169276)Anti-GM130 antibody - BSA and Azide free (ab169276) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 112 kDa -
Western blot - Anti-GM130 antibody - BSA and Azide free (ab169276)All lanes : Anti-GM130 antibody - BSA and Azide free (ab169276) at 1 µg/ml
Lane 1 : GM130 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 112 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab169276 has been referenced in 20 publications.
- Yang Z et al. Exosomes derived from cancer stem cells of gemcitabine-resistant pancreatic cancer cells enhance drug resistance by delivering miR-210. Cell Oncol (Dordr) 43:123-136 (2020). PubMed: 31713003
- Singh S et al. Mapping the Neuroanatomy of ABHD16A, ABHD12, and Lysophosphatidylserines Provides New Insights into the Pathophysiology of the Human Neurological Disorder PHARC. Biochemistry 59:2299-2311 (2020). PubMed: 32462874
- McHowat J et al. 2-Chlorofatty Aldehyde Elicits Endothelial Cell Activation. Front Physiol 11:460 (2020). PubMed: 32457656
- Xia Y et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther 11:313 (2020). PubMed: 32698909
- Frisbie CP et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells 8:N/A (2019). PubMed: 31847122