Anti-GM130 antibody - BSA and Azide free (ab169276)
Key features and details
- Mouse polyclonal to GM130 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GM130 antibody - BSA and Azide free
See all GM130 primary antibodies -
Description
Mouse polyclonal to GM130 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GM130 aa 1-1000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
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ICC/IF | (4) |
Use a concentration of 7 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa. |
ICC/IF
Use a concentration of 7 µg/ml. |
Target
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Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
Sequence similarities
Belongs to the GOLGA2 family. -
Domain
Extended rod-like protein with coiled-coil domains. -
Cellular localization
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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Database links
- Entrez Gene: 2801 Human
- Omim: 602580 Human
- SwissProt: Q08379 Human
- Unigene: 155827 Human
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Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Images
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for GM130 (green) using ab169276 at 7 µg/ml in ICC/IF.
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Anti-GM130 antibody - BSA and Azide free (ab169276) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 112 kDa -
All lanes : Anti-GM130 antibody - BSA and Azide free (ab169276) at 1 µg/ml
Lane 1 : GM130 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 112 kDa
Protocols
Datasheets and documents
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Datasheet download
References (28)
ab169276 has been referenced in 28 publications.
- Piovesana R et al. Notch Signal Mediates the Cross-Interaction between M2 Muscarinic Acetylcholine Receptor and Neuregulin/ErbB Pathway: Effects on Schwann Cell Proliferation. Biomolecules 12:N/A (2022). PubMed: 35204740
- Xie Z et al. Structural insights into a shared mechanism of human STING activation by a potent agonist and an autoimmune disease-associated mutation. Cell Discov 8:133 (2022). PubMed: 36513640
- Li P et al. Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites. Mol Biol Cell 32:422-434 (2021). PubMed: 33405953
- Lin H et al. Nerve growth factor regulates liver cancer cell polarity and motility. Mol Med Rep 23:N/A (2021). PubMed: 33649819
- Hiller H et al. Altered cellular localisation and expression, together with unconventional protein trafficking, of prion protein, PrPC, in type 1 diabetes. Diabetologia 64:2279-2291 (2021). PubMed: 34274990