Product nameAnti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker
See all GM130 primary antibodies
DescriptionRabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Drosophila melanogaster
- Drosophila S2 Cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab30637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).|
FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Sequence similaritiesBelongs to the GOLGA2 family.
DomainExtended rod-like protein with coiled-coil domains.
Cellular localizationGolgi apparatus > Golgi stack membrane.
- Information by UniProt
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml
Lane 1 : Drosophila melanogaster S2 cell extract
Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 95 kDa
Observed band size: 95 kDa
ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.
All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml
Lane 1 :
Schneider L2 whole cell lysate (ab14893)
Lane 2 :
Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 95 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Gerards M et al. Intracellular vesicle trafficking plays an essential role in mitochondrial quality control. Mol Biol Cell N/A:N/A (2018). Read more (PubMed: 29343549) »
- Gordon DE et al. VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane. PLoS Genet 13:e1006698 (2017). Read more (PubMed: 28403141) »