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    gm130-antibody-ep892y-cis-golgi-marker-ab52649.pdf

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RecombinantRabMAb

Recombinant Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

  • Datasheet
  • SDS
Reviews (38)Q&A (4)References (203)

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Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
  • Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB, IP
  • Reacts with: Dog, Human, African green monkey

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-GM130 antibody [EP892Y] - cis-Golgi Marker
    See all GM130 primary antibodies
  • Description

    Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • Host species

    Rabbit
  • Specificity

    Mouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Dog, Human, African green monkey
    Predicted to work with: Cow, MonkeyDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GM130 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q08379

  • Positive control

    • WB: HeLa, MCF7, MDCK(NBL-2), MDBK(BL-1) and COS-1 cell lysates; MDCK 2 cell lysate; COS-7 cell lysate. IHC-P: Human cervix carcinoma and liver tissues. ICC/IF: HeLa and ARPE-19 cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP892Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
    • Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    • Alexa Fluor® 488 Anti-GM130 antibody [EP892Y] (ab275987)
    • Alexa Fluor® 594 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab277236)
    • Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab277924)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human GM130 protein (ab131818)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Golgi Staining Kit - Green Fluorescence - Cytopainter (ab139483)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.
ICC/IF (20)
1/50 - 1/250.

PFA fixation should be most suitable.

IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Overnight incubation is recommended.

WB (11)
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
IP
1/20 - 1/50.
Notes
Flow Cyt (Intra)
1/20.
ICC/IF
1/50 - 1/250.

PFA fixation should be most suitable.

IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Overnight incubation is recommended.

WB
1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
IP
1/20 - 1/50.

Target

  • Function

    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • Sequence similarities

    Belongs to the GOLGA2 family.
  • Domain

    Extended rod-like protein with coiled-coil domains.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Target information above from: UniProt accession Q08379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2801 Human
    • Omim: 602580 Human
    • SwissProt: Q08379 Human
    • Unigene: 155827 Human
    • Alternative names

      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • Gm130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • Golga2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GM130 with purified ab52649 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      ab52649 (purified) at 1/20 immunoprecipitating GM130 in HeLa whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.



      All lanes :

      Lane 1 : HeLa whole cell lysate at 10 µg
      Lane 2 : ab52649 + HeLa whole cell lysate at 10 µg
      Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab52649 in HeLa whole cell lysate

      Observed band size: 130 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling GM130 with purified ab52649 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      All lanes : Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

      Unpurified ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

      See Abreview

    • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/200000 dilution (unpurified) + HeLa cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 112 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      ICC/IF image of unpurified ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Flow Cytometry (Intracellular) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GM130 (red) with ab52649 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GM130 with unpurified ab52649 at a dilution of 1/500.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      All lanes : Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) at 1/5000 dilution (purified)

      Lane 1 : MDCK(NBL-2) cell lysate
      Lane 2 : MDCK(BL-1) cell lysate
      Lane 3 : COS-1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 112 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      MDCK 2 cells at 25 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 112 kDa



      Blocking and dilution buffer: 1XPBS-Tween, 5% milk

      Exposure: 10 minutes.

      See Abreview

    • Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Western blot - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      COS-7 Cell Line from African green monkey kidney whole cell lysate

      Predicted band size: 112 kDa



      Blocking and dilution buffer: PBS, 0.05% Tween 

      Exposure: 5 minutes.

      See Abreview

    • Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
      Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (203)

    Publishing research using ab52649? Please let us know so that we can cite the reference in this datasheet.

    ab52649 has been referenced in 203 publications.

    • Arab T  et al. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms. J Extracell Vesicles 10:e12079 (2021). PubMed: 33850608
    • Chhoy P  et al. Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models. STAR Protoc 2:100303 (2021). PubMed: 33554138
    • Chatterjee Bhowmick D  et al. FoxA2 and RNA Pol II mediate human islet amyloid polypeptide turnover in ER-stressed pancreatic ß-cells. Biochem J 478:1261-1282 (2021). PubMed: 33650632
    • Migazzi A  et al. Huntingtin-mediated axonal transport requires arginine methylation by PRMT6. Cell Rep 35:108980 (2021). PubMed: 33852844
    • Huang M  et al. Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D. Mol Metab 47:101182 (2021). PubMed: 33549845
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 42 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney(BS-C-1 cells))
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Kidney(BS-C-1 cells)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Read More

    Mr. J. Y. Seo

    Verified customer

    Submitted Mar 28 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Vero cells)
    Permeabilization
    Yes - Saponin
    Specification
    Vero cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 25 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human fibroblasts)
    Permeabilization
    Yes - 0.1% Triton in PBS (5 mins)
    Specification
    Human fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Tom Ryan

    Verified customer

    Submitted Sep 29 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse embrional fibroblasts)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    Mouse embrional fibroblasts
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Apr 11 2019

    Western blot abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse adult brain)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    Mouse adult brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Apr 11 2019

    Western blot abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Apr 02 2019

    Western blot abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (COS-7 Cell Line from African green monkey kidney)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    25 µg
    Specification
    COS-7 Cell Line from African green monkey kidney
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Dec 18 2018

    Western blot abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human primary dermal fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Human primary dermal fibroblasts
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Dec 10 2018

    Western blot abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Tissue lysate - whole (heart)
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain tissue section:IHC-FoFr (PFA perfusion fixed)
    Specification
    brain tissue section:IHC-FoFr (PFA perfusion fixed
    Fixative
    Formaldehyde
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    Submitted Jan 31 2018

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