Anti-GM2A antibody (ab113416)

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Overview

  • Product name

    Anti-GM2A antibody
    See all GM2A primary antibodies

  • Description

    Goat polyclonal to GM2A

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human GM2A aa 179-189 (internal sequence).
    Sequence:

    KRLGCIKIAAS


    Database link: NP_000396.2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human GM2A protein (ab116790) can be used as a positive control in WB. HEK293 lysate

  • General notes

     This product was previously labelled as GM2-AP

     

Properties

Applications

Our Abpromise guarantee covers the use of ab113416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
ELISA 1/4000.

Target

  • Function

    Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3.

  • Involvement in disease

    Defects in GM2A are the cause of GM2-gangliosidosis type AB (GM2GAB) [MIM:272750]; also known as Tay-Sachs disease AB variant. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2GAB is characterized by GM2 gangliosides accumulation in the presence of both hexosaminidase A and B.

  • Post-translational
    modifications

    The serines in positions 32 and 33 are absent in 80% of the sequenced protein.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cerebroside sulfate activator protein antibody
    • ganglioside GM2 activator antibody
    • Ganglioside GM2 activator isoform short antibody
    • Ganglioside GM2 activator precursor antibody
    • GM2 activator antibody
    • GM2 AP antibody
    • GM2 ganglioside activator antibody
    • GM2 ganglioside activator protein antibody
    • GM2-AP antibody
    • GM2A antibody
    • GM2AP antibody
    • OTTHUMP00000160619 antibody
    • SAP 3 antibody
    • SAP-3 antibody
    • SAP3 antibody
    • SAP3_HUMAN antibody
    • Shingolipid activator protein 3 antibody
    • Sphingolipid activator protein 3 antibody
    see all

Images

  • Western blot - Anti-GM2A antibody (ab113416)
    Western blot - Anti-GM2A antibody (ab113416)
    Lane 1 : Anti-GM2A antibody (ab113416) at 1 µg/ml
    Lane 2 : Anti-GM2A antibody (ab113416) at 1 mg/ml

    Lane 1 : HEK293 lysate overexpressing Human GM2A
    Lane 2 : Mock-transfected HEK293

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa



    Primary incubations were for 1 hour. Detected by chemiluminescence.

References

ab113416 has not yet been referenced specifically in any publications.

Publishing research using ab113416? Please let us know so that we can cite the reference in this datasheet.

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