Key features and details
- Rabbit polyclonal to GM2A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GM2A antibody
See all GM2A primary antibodies
DescriptionRabbit polyclonal to GM2A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: RT4 cell lysate. IHC-P: Human kidney tissue.
This product was previously labelled as GM2-AP
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.|
FunctionBinds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3.
Involvement in diseaseDefects in GM2A are the cause of GM2-gangliosidosis type AB (GM2GAB) [MIM:272750]; also known as Tay-Sachs disease AB variant. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2GAB is characterized by GM2 gangliosides accumulation in the presence of both hexosaminidase A and B.
modificationsThe serines in positions 32 and 33 are absent in 80% of the sequenced protein.
- Information by UniProt
- Cerebroside sulfate activator protein antibody
- ganglioside GM2 activator antibody
- Ganglioside GM2 activator isoform short antibody
All lanes : Anti-GM2A antibody (ab224246) at 0.4 µg/ml
Lane 1 : SK-MEL-30 cell lysates
Lane 2 : MCF-7 cell lysates
Predicted band size: 21 kDa
Loading control: Anti-PFN1.
Immunohistochemical analysis of paraffin-embedded human skin tissue staining GM2A with ab224246 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue staining GM2A with ab224246 at 1/200 dilution.
Paraffin embedded human kidney tissue stained for GM2A with ab224246 (1/200 dilution) in immunohistochemical analysis.
Anti-TRIOBP antibody (ab224226) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 21 kDa
ab224246 has not yet been referenced specifically in any publications.