Key features and details
- Rabbit polyclonal to GMAP-210
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GMAP-210 antibody
See all GMAP-210 primary antibodies
DescriptionRabbit polyclonal to GMAP-210
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human GMAP-210 aa 25-75.
- Whole cell lysates from HeLa and 293T cells.
This product was previously labelled as TRIP230
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72223 was affinity purified using an epitope specific to GMAP-210 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 228 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceTRIP230 was identified through its ability to interact functionally with thyroid hormone receptor-beta. It has also been found in association with the Golgi apparatus and microtubules. TRIP230 binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. This protein is probably involved in maintaining cis-Golgi structure.
Cellular localizationCell Membrane and Golgi Apparatus
- CEV 14 antibody
- CEV14 antibody
- Clonal evolution related gene on chromosome 14 antibody
All lanes : Anti-GMAP-210 antibody (ab72223) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 228 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Detection of Human GMAP-210 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with ab72223 at 3µg in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Image shows immunoprecipitated GMAP-210 detected with post IP WB, loading 20% of IP and using ab72223 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab72223 has been referenced in 1 publication.
- Snyder JC et al. Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network. J Biol Chem 288:10286-97 (2013). PubMed: 23439653