Key features and details
- Rabbit polyclonal to GMDS
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GMDS antibody
See all GMDS primary antibodies
DescriptionRabbit polyclonal to GMDS
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human GMDS aa 322-372. The exact sequence is proprietary.
Database link: O60547
- IP: HEK-293T whole cell lysate.
ab242051 has not performed satisfactorily when used for WB of GMDS in crude preparations (e.g. whole cell lysate).
This antibody can be used for WB of enriched (e.g.immunoprecipitated) sources of GMDS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to GMDS immobilized on solid support.
Our Abpromise guarantee covers the use of ab242051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionConversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose.
PathwayNucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Sequence similaritiesBelongs to the GDP-mannose 4,6-dehydratase family.
- Information by UniProt
- GDP D mannose dehydratase antibody
- GDP mannose 4 6 dehydratase antibody
- GDP-D-mannose dehydratase antibody
GMDS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242051 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab242051 at 1 µg/mL.
Lane 1: ab242051 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242051 has not yet been referenced specifically in any publications.