Overview

  • Product name

  • Description

    Rabbit polyclonal to GNA12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GNA12 aa 112-270.
    Database link: Q03113

  • Positive control

    • WB: HeLa, MCF7, HepG2 and A549 whole cell lysate. IHC-P: Human liver cancer and placenta tissue. ICC/IF: HeLa cells.
  • General notes

    Protein previously labeled as G protein alpha 12.

     This product was previously labelled as G alpha-12

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
  • Sequence similarities

    Belongs to the G-alpha family. G(12) subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • G alpha 12 antibody
    • G alpha-12 antibody
    • G-protein subunit alpha-12 antibody
    • gep antibody
    • Gna12 antibody
    • GNA12_HUMAN antibody
    • guanine nucleotide binding protein (G protein) alpha 12 antibody
    • Guanine nucleotide binding protein alpha 12 subunit antibody
    • Guanine nucleotide-binding protein subunit alpha-12 antibody
    • MGC104623 antibody
    • MGC99644 antibody
    • NNX3 antibody
    • RMP antibody
    • WUGSC:H_GS165O14.2 antibody
    see all

Images

  • All lanes : Anti-GNA12 antibody (ab236617) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for GNA12 using ab236617 at a dilution of 1/133 in ICC/IF.
    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human liver cancer tissue stained for GNA12 with ab236617 at a 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human placenta tissue stained for GNA12 with ab236617 at a 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab236617 has not yet been referenced specifically in any publications.

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