Overview

  • Product name

  • Description

    Rabbit polyclonal to GNA13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human GNA13 (internal sequence). The exact sequence is proprietary.
    Database link: Q14344

  • Positive control

    • WB: HeLa, HEK-293T, A431 and HepG2 whole cell extracts.
  • General notes

     This product was previously labelled as G protein alpha 13

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
  • Sequence similarities

    Belongs to the G-alpha family. G(12) subfamily.
  • Post-translational
    modifications

    Palmitoylation is critical for proper membrane localization and signaling.
    Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • G alpha 13 antibody
    • G alpha-13 antibody
    • G-protein subunit alpha-13 antibody
    • GNA13 antibody
    • GNA13_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha 13 antibody
    • Guanine nucleotide binding protein alpha 13 subunit antibody
    • Guanine nucleotide-binding protein subunit alpha-13 antibody
    see all

Images

  • All lanes : Anti-GNA13 antibody (ab227494) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : G protein alpha 13 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 44 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-GNA13 antibody (ab227494) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 44 kDa



    10% SDS-PAGE gel.

References

ab227494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227494.
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