Anti-GNA13 antibody (ab246892)

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Overview

  • Product name

    Anti-GNA13 antibody
    See all GNA13 primary antibodies

  • Description

    Rabbit polyclonal to GNA13

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human GNA13 aa 72-173.
    Sequence:

    HGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDK MMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLG ES


    Database link: Q14344

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: human stomach tissue. ICC/IF: SH-SY5Y cells.

  • General notes

     This product was previously labelled as G protein alpha 13

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

  • Sequence similarities

    Belongs to the G-alpha family. G(12) subfamily.

  • Post-translational
    modifications

    Palmitoylation is critical for proper membrane localization and signaling.
    Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.

  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • G alpha 13 antibody
      • G alpha-13 antibody
      • G-protein subunit alpha-13 antibody
      • GNA13 antibody
      • GNA13_HUMAN antibody
      • Guanine nucleotide binding protein (G protein) alpha 13 antibody
      • Guanine nucleotide binding protein alpha 13 subunit antibody
      • Guanine nucleotide-binding protein subunit alpha-13 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GNA13 antibody (ab246892)
      Immunocytochemistry/ Immunofluorescence - Anti-GNA13 antibody (ab246892)

      PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for GNA13 (green) using ab246892 at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody (ab246892)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody (ab246892)

      Paraffin-embedded human stomach tissue stained for GNA13 using ab246892 at 1/50 dilution in immunohistochemical analysis.

    References

    ab246892 has not yet been referenced specifically in any publications.

    Publishing research using ab246892? Please let us know so that we can cite the reference in this datasheet.

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