Overview

  • Product name

    Anti-GNA13 antibody [EPR5436]
    See all GNA13 primary antibodies
  • Description

    Rabbit monoclonal [EPR5436] to GNA13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GNA13 aa 300 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Y79, 293T, and HepG2 cell lysates; Human bladder carcinoma and kidney tissues
  • General notes

     This product was previously labelled as G protein alpha 13

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5436
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250-1/500. 

See IHC antigen retrieval protocol.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
    • Sequence similarities

      Belongs to the G-alpha family. G(12) subfamily.
    • Post-translational
      modifications

      Palmitoylation is critical for proper membrane localization and signaling.
      Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.
    • Cellular localization

      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • G alpha 13 antibody
      • G alpha-13 antibody
      • G-protein subunit alpha-13 antibody
      • GNA13 antibody
      • GNA13_HUMAN antibody
      • Guanine nucleotide binding protein (G protein) alpha 13 antibody
      • Guanine nucleotide binding protein alpha 13 subunit antibody
      • Guanine nucleotide-binding protein subunit alpha-13 antibody
      see all

    Images

    • All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution (unpurified)

      Lane 1 : Y79 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution (purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution (unpurified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 44 kDa
      Observed band size: 44 kDa


      Exposure time: 30 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kim JE  et al. Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling. Int J Mol Med 43:185-198 (2019). Read more (PubMed: 30387811) »
    • Muhammad S  et al. miRNA-30d serves a critical function in colorectal cancer initiation, progression and invasion via directly targeting the GNA13 gene. Exp Ther Med 17:260-272 (2019). Read more (PubMed: 30651791) »
    See all 6 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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