Overview

  • Product name

    Anti-GNAI1 + GNAI2 antibody
  • Description

    Rabbit polyclonal to GNAI1 + GNAI2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish
  • Immunogen

    Synthetic peptide corresponding to Human GNAI1 + GNAI2 aa 346-355.
    Sequence:

    KNNLKDCGLF


    (Peptide available as ab5006)

  • General notes

     This product was previously labelled as G protein alpha inhibitor 1, G Protein alpha Inhibitor 1+2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa.Can be blocked with GNAI1 + GNAI2 peptide (ab5006).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 1 µg/ml.

Target

Images

  • IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Vosahlikova M  et al. Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of d-opioid receptor amount and function. Biochem Pharmacol 154:452-463 (2018). Read more (PubMed: 29883706) »
  • Cechova K  et al. Up-regulation of µ-, d- and ?-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes. J Neuroimmunol 321:12-23 (2018). Read more (PubMed: 29957382) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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