GNAI1 + GNAI2 peptide (ab5006)
Key features and details
- Purity: > 70% HPLC
- Suitable for: Blocking
Description
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Product name
GNAI1 + GNAI2 peptide -
Purity
> 70 % HPLC.
Peptides are analyzed by Reverse-Phase HPLC (RP-HPLC) in order to determine purity. Identities are confirmed by MALDI-MS. -
Animal free
No -
Nature
Synthetic
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab5006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Blocking
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Form
Lyophilized -
Additional notes
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human G protein alpha inhibitor 1/2, catalog ab3522. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.
This product was previously labelled as G protein alpha inhibitor 1, G Protein alpha Inhibitor 1+2
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Reconstitution>95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.
General Info
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Alternative names
- Adenylate cyclase-inhibiting G alpha protein
- Gi
- Gi1 protein alpha subunit
see all -
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab5006 has not yet been referenced specifically in any publications.