Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment within Human GNAI1 (internal sequence). The exact sequence is proprietary.
    Database link: P63096

  • Positive control

    • WB: A431 and Hela S3 whole cell lysates. IHC-P: Human colon carcinoma tissue.
  • General notes

     This product was previously labelled as G protein alpha inhibitor 1, G Protein alpha Inhibitor 1+2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase inhibitory protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • G protein alpha inhibiting 1 antibody
    • G protein alpha inhibiting activity polypeptide 1 antibody
    • Gi antibody
    • Gi inhibitory G protein antibody
    • Gi1 protein alpha subunit antibody
    • GNAI 1 antibody
    • GNAI1 antibody
    • GNAI1_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1 antibody
    • Guanine nucleotide binding protein alpha inhibiting activity polypeptide 1 antibody
    • Guanine nucleotide binding protein G(i) alpha 1 subunit antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-1 antibody
    • OTTHUMP00000161319 antibody
    • OTTHUMP00000208047 antibody
    see all

Images

  • All lanes : Anti-GNAI1 antibody (ab228623) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for GNAI1 using ab228623 at 1/500 dilution in immunohistochemical analysis.

References

ab228623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228623.
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