Key features and details
- Rabbit polyclonal to GNAI1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GNAI1 antibody
See all GNAI1 primary antibodies
DescriptionRabbit polyclonal to GNAI1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Zebrafish
Recombinant fragment within Human GNAI1 (internal sequence). The exact sequence is proprietary.
Database link: P63096
- WB: A431 and Hela S3 whole cell lysates. IHC-P: Human colon carcinoma tissue.
This product was previously labelled as G protein alpha inhibitor 1, G Protein alpha Inhibitor 1+2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
Cellular localizationNucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.
- Information by UniProt
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase inhibitory protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
All lanes : Anti-GNAI1 antibody (ab228623) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
12% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for GNAI1 using ab228623 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228623 has not yet been referenced specifically in any publications.