Recombinant Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9441(B)] to GNAI1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GNAI1 antibody [EPR9441(B)]
See all GNAI1 primary antibodies -
Description
Rabbit monoclonal [EPR9441(B)] to GNAI1 -
Host species
Rabbit -
Specificity
The immunogenic sequence of ab140125 has 92.9% homology with GNAI3 and 64% (4 continuous residues) homology with GNAI2 which might cause cross reactivity with GNAI3 and GNAI2. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- SH-SY5Y, U-87 MG, Human glioma, and Human fetal brain lysates; Human endometrial carcinoma and thyroid carcinoma tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9441(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/999 - 1/9999.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt (Intra)
1/999 - 1/9999. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody. - Information by UniProt
-
Database links
- Entrez Gene: 2770 Human
- Entrez Gene: 14677 Mouse
- Entrez Gene: 25686 Rat
- Omim: 139310 Human
- SwissProt: P63096 Human
- SwissProt: B2RSH2 Mouse
- SwissProt: P10824 Rat
- Unigene: 134587 Human
see all -
Alternative names
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase inhibitory protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
see all
Images
-
All lanes : Anti-GNAI1 antibody [EPR9441(B)] (ab140125) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : Human glioma tissue lysate
Lane 4 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 40 kDa -
Overlay histogram showing SHSY-5Y cells stained with ab140125 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140125, 1/9999 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling GNAI1 with ab140125 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab140125 has been referenced in 3 publications.
- Chen JJ et al. DHHC5 Mediates ß-Adrenergic Signaling in Cardiomyocytes by Targeting Ga Proteins. Biophys J 118:826-835 (2020). PubMed: 31547976
- Touhara KK & MacKinnon R Molecular basis of signaling specificity between GIRK channels and GPCRs. Elife 7:N/A (2018). PubMed: 30526853
- Streit MR et al. Cardiac Effects of Attenuating Gsa - Dependent Signaling. PLoS One 11:e0146988 (2016). WB . PubMed: 26811901