Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab20392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
- Information by UniProt
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
- GIP antibody
Lanes 1 & 3 : Anti-GNAI2 antibody (ab20392) at 1/1000 dilution
Lane 2 : No primary
All lanes : Rat RH7777 whole cell lysate
Lysates/proteins at 15 µg per lane.
Lane 1 : No secondary
Lanes 2-3 : Alkaline phosphatase conjugated goat anti-rabbit
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 10 minutes
This product has been referenced in:
- Ramírez VT et al. Wnt-5a/Frizzled9 Receptor Signaling through the Gao-Gß? Complex Regulates Dendritic Spine Formation. J Biol Chem 291:19092-107 (2016). Read more (PubMed: 27402827) »
- Fu X et al. MicroRNA-222-3p/GNAI2/AKT axis inhibits epithelial ovarian cancer cell growth and associates with good overall survival. Oncotarget 7:80633-80654 (2016). WB ; Human . Read more (PubMed: 27811362) »