Product nameAnti-GNAI2 antibody [EPR9468]
See all GNAI2 primary antibodies
DescriptionRabbit monoclonal [EPR9468] to GNAI2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human GNAI2 aa 50-150.
Database link: P04899
- HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
- Information by UniProt
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
- GIP antibody
All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : U-87 MG cell lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 40 kDa
Flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).
ab137050 has not yet been referenced specifically in any publications.