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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins
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RecombinantRabMAb

Recombinant Anti-GNAI2 antibody [EPR9468] (ab137050)

  • Datasheet
  • SDS
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Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
  • Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)
  • Anti-GNAI2 antibody [EPR9468] (ab137050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9468] to GNAI2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human GNAI2 protein (ab132215)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GNAI2 antibody [EPR9468]
    See all GNAI2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9468] to GNAI2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human GNAI2 aa 50-150.
    Database link: P04899

  • Positive control

    • HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells
  • General notes

     

     

     This product was previously labelled as G protein alpha Inhibitor 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9468
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins

Associated products

  • Alternative Versions

    • Anti-GNAI2 antibody [EPR9468] - BSA and Azide free (ab248787)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • U937 cytoplasmic extract lysate (ab14904)
    • MCF7 membrane extract lysate (ab29539)
    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
    • RAW 264.7 whole cell lysate (ab7187)
    • HL-60 whole cell lysate (ab7914)
  • Recombinant Protein

    • Recombinant Human GNAI2 protein (ab132215)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
    • Sequence similarities

      Belongs to the G-alpha family. G(i/o/t/z) subfamily.
    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Target information above from: UniProt accession P04899 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2771 Human
      • Entrez Gene: 14678 Mouse
      • Entrez Gene: 81664 Rat
      • Omim: 139360 Human
      • SwissProt: P04899 Human
      • SwissProt: P08752 Mouse
      • SwissProt: P04897 Rat
      • Unigene: 77269 Human
      • Unigene: 196464 Mouse
      • Unigene: 3036 Rat
      see all
    • Alternative names

      • Adenylate cyclase inhibiting G alpha protein antibody
      • Adenylate cyclase-inhibiting G alpha protein antibody
      • GIP antibody
      • GNAI 2 antibody
      • Gnai2 antibody
      • GNAI2_HUMAN antibody
      • GNAI2B antibody
      • GTP binding regulatory protein Gi alpha 2 chain antibody
      • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
      • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
      • H LUCA15.1 antibody
      • H LUCA16.1 antibody
      • WUGSC:H LUCA15.1 antibody
      • WUGSC:H LUCA16.1 antibody
      see all

    Images

    • Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : U-87 MG cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 40 kDa

    • Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)

      Flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).

    • Anti-GNAI2 antibody [EPR9468] (ab137050)
      Anti-GNAI2 antibody [EPR9468] (ab137050)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab137050? Please let us know so that we can cite the reference in this datasheet.

    ab137050 has not yet been referenced specifically in any publications.

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