Recombinant
RabMAb

Recombinant Anti-GNAI2 antibody [EPR9468] (ab137050)

Submit a review Submit a question

Overview

  • Product name

    Anti-GNAI2 antibody [EPR9468]
    See all GNAI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9468] to GNAI2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human GNAI2 aa 50-150.
    Database link: P04899

  • Positive control

    • HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells

  • General notes

     

     

     This product was previously labelled as G protein alpha Inhibitor 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

    • Sequence similarities

      Belongs to the G-alpha family. G(i/o/t/z) subfamily.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adenylate cyclase inhibiting G alpha protein antibody
      • Adenylate cyclase-inhibiting G alpha protein antibody
      • GIP antibody
      • GNAI 2 antibody
      • Gnai2 antibody
      • GNAI2_HUMAN antibody
      • GNAI2B antibody
      • GTP binding regulatory protein Gi alpha 2 chain antibody
      • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
      • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
      • H LUCA15.1 antibody
      • H LUCA16.1 antibody
      • WUGSC:H LUCA15.1 antibody
      • WUGSC:H LUCA16.1 antibody
      see all

    Images

    • Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : U-87 MG cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 40 kDa

    • Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)

      Flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).

    References

    ab137050 has not yet been referenced specifically in any publications.

    Publishing research using ab137050? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab137050.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com