RecombinantRabMAb

Recombinant Anti-GNAI2 antibody [EPR9468] (ab137050)

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Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
  • Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)
  • Anti-GNAI2 antibody [EPR9468] (ab137050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9468] to GNAI2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GNAI2 antibody [EPR9468]
    See all GNAI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9468] to GNAI2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human GNAI2 aa 50-150.
    Database link: P04899

  • Positive control

    • HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells

  • General notes

     

     

     This product was previously labelled as G protein alpha Inhibitor 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9468

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

    • Sequence similarities

      Belongs to the G-alpha family. G(i/o/t/z) subfamily.

    • Cellular localization

      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adenylate cyclase inhibiting G alpha protein antibody
      • Adenylate cyclase-inhibiting G alpha protein antibody
      • GIP antibody
      • GNAI 2 antibody
      • Gnai2 antibody
      • GNAI2_HUMAN antibody
      • GNAI2B antibody
      • GTP binding regulatory protein Gi alpha 2 chain antibody
      • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
      • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
      • H LUCA15.1 antibody
      • H LUCA16.1 antibody
      • WUGSC:H LUCA15.1 antibody
      • WUGSC:H LUCA16.1 antibody
      see all

    Images

    • Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)
      All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : U-87 MG cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 40 kDa

    • Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)
      Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)

      Flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).

    • Anti-GNAI2 antibody [EPR9468] (ab137050)
      Anti-GNAI2 antibody [EPR9468] (ab137050)

    References (0)

    Publishing research using ab137050? Please let us know so that we can cite the reference in this datasheet.

    ab137050 has not yet been referenced specifically in any publications.

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