Recombinant Anti-GNAI2 antibody [EPR9468] (ab137050)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GNAI2 antibody [EPR9468]
See all GNAI2 primary antibodies -
Description
Rabbit monoclonal [EPR9468] to GNAI2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
corresponding to Human GNAI2 aa 50-150.
Database link: P04899 -
Positive control
- HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells
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General notes
This product was previously labelled as G protein alpha Inhibitor 2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9468 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
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Database links
- Entrez Gene: 2771 Human
- Entrez Gene: 14678 Mouse
- Entrez Gene: 81664 Rat
- Omim: 139360 Human
- SwissProt: P04899 Human
- SwissProt: P08752 Mouse
- SwissProt: P04897 Rat
- Unigene: 77269 Human
see all -
Alternative names
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
- GIP antibody
see all
Images
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![Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)](https://www.abcam.com/ps/products/137/ab137050/Images/ab137050-151548-anti-g-protein-alpha-inhibitor-2-antibody-epr9468-western-blot.jpg)
Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050)All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : U-87 MG cell lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 40 kDa -
![Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)](https://www.abcam.com/ps/products/137/ab137050/Images/ab137050-151547-anti-g-protein-alpha-inhibitor-2-antibody-epr9468-flow-cytometry.jpg)
Flow Cytometry - Anti-GNAI2 antibody [EPR9468] (ab137050)Flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).
Datasheets and documents
References
ab137050 has not yet been referenced specifically in any publications.
