Recombinant Anti-GNAI2 antibody [EPR9468] (ab137050)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9468] to GNAI2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-GNAI2 antibody [EPR9468]
See all GNAI2 primary antibodies -
Description
Rabbit monoclonal [EPR9468] to GNAI2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HL60, U937, MCF7, HeLa, U-87 MG, C6 and RAW 264.7 cell lysates; HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9468 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
-
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
-
Database links
- Entrez Gene: 2771 Human
- Entrez Gene: 14678 Mouse
- Entrez Gene: 81664 Rat
- Omim: 139360 Human
- SwissProt: P04899 Human
- SwissProt: P08752 Mouse
- SwissProt: P04897 Rat
- Unigene: 77269 Human
see all -
Alternative names
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
- GIP antibody
see all
Images
-
All lanes : Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : U-87 MG cell lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 40 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab137050 has been referenced in 1 publication.
- Seo JH & Jeon YJ Global Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells via Connective Tissue Growth Factor Treatment under Chemically Defined Feeder-Free Culture Conditions. J Microbiol Biotechnol 32:126-140 (2022). PubMed: 34750284