Overview

  • Product name

    Anti-GNAI2 antibody [EPR9469] (HRP)
    See all GNAI2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9469] to GNAI2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GNAI2. The exact sequence is proprietary.
    Database link: P04899
    (Peptide available as ab226769)

  • Positive control

    • WB: Human Fetal Brain tissue lysate, U-87 MG cell lysate. IHC-P: FFPE human kidney (normal) tissue sections.
  • General notes

     This product was previously labelled as G protein alpha Inhibitor 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9469
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human ENO1 peptide (ab226769).
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • GIP antibody
    • GNAI 2 antibody
    • Gnai2 antibody
    • GNAI2_HUMAN antibody
    • GNAI2B antibody
    • GTP binding regulatory protein Gi alpha 2 chain antibody
    • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
    • H LUCA15.1 antibody
    • H LUCA16.1 antibody
    • WUGSC:H LUCA15.1 antibody
    • WUGSC:H LUCA16.1 antibody
    see all

Images

  • All lanes : Anti-GNAI2 antibody [EPR9469] (HRP) (ab205782) at 1/5000 dilution

    Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205782 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of GNAI2 staining in a section of formalin-fixed paraffin-embedded normal human kidney tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205782, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab205782 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205782.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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