Anti-GNAI3 antibody (ab14246)

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Western blot - Anti-GNAI3 antibody (ab14246)
  • Immunocytochemistry/ Immunofluorescence - Anti-GNAI3 antibody (ab14246)

Key features and details

  • Chicken polyclonal to GNAI3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Guinea pig, Human
  • Isotype: IgY

Overview

  • Product name

    Anti-GNAI3 antibody
    See all GNAI3 primary antibodies

  • Description

    Chicken polyclonal to GNAI3

  • Host species

    Chicken

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Guinea pig, Human
    Predicted to work with: Hamster

  • Immunogen

    KNNLKECGLY

    , corresponding to amino acids 345-354 of GAI3.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human GNAI3 protein (ab124586) can be used as a positive control in WB. AtT 20 and Normal rat kidney (NRK) cell line.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab14246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF 1/200.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.

  • Cellular localization

    Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 87U6 antibody
    • FLJ26559 antibody
    • G protein alpha inhibiting 3 antibody
    • G(i) alpha 3 antibody
    • G(i) alpha-3 antibody
    • GNAI3 antibody
    • GNAI3_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody
    • Guanine nucleotide binding protein G(k) alpha subunit antibody
    • Guanine nucleotide-binding protein G(k) subunit alpha antibody
    • OTTHUMP00000013368 antibody
    see all

Images

  • Western blot - Anti-GNAI3 antibody (ab14246)
    Western blot - Anti-GNAI3 antibody (ab14246)

    Western blot detection of endogenous GAI3 protein in normal rat kidney (NRK) cell line using ab14246.

  • Immunocytochemistry/ Immunofluorescence - Anti-GNAI3 antibody (ab14246)
    Immunocytochemistry/ Immunofluorescence - Anti-GNAI3 antibody (ab14246)

    Immunocytochemistry staining of endogenous GAI3 protein in AtT 20 (Left) and normal rat kidney (NRK) (right) cell lines. ab14246 dilution: 1/200; staining using Alexa conjugated secondary antibody.

References (2)

Publishing research using ab14246? Please let us know so that we can cite the reference in this datasheet.

ab14246 has been referenced in 2 publications.

  • Freeman S  et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879
  • Bhonker Y  et al. The GPSM2/LGN GoLoco motifs are essential for hearing. Mamm Genome 27:29-46 (2016). PubMed: 26662512

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