Anti-GNAI3 antibody (ab14246)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-GNAI3 antibody
See all GNAI3 primary antibodies -
DescriptionChicken polyclonal to GNAI3
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Host speciesChicken
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Tested applicationsSuitable for: ICC, WBmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Guinea pig, Human
Predicted to work with: Hamster
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Immunogen
KNNLKECGLY
, corresponding to amino acids 345-354 of GAI3. -
Positive control
- Recombinant Human GNAI3 protein (ab124586) can be used as a positive control in WB. AtT 20 and Normal rat kidney (NRK) cell line.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab14246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/200. | |
| WB | 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
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FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
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Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
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Cellular localizationCytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
- Information by UniProt
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Database links
- Entrez Gene: 374097 Chicken
- Entrez Gene: 2773 Human
- Entrez Gene: 14679 Mouse
- Entrez Gene: 25643 Rat
- Omim: 139370 Human
- SwissProt: P38403 Guinea pig
- SwissProt: P08754 Human
- SwissProt: Q9DC51 Mouse
see all -
Alternative names
- 87U6 antibody
- FLJ26559 antibody
- G protein alpha inhibiting 3 antibody
see all
Images
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Western blot - Anti-GNAI3 antibody (ab14246)Western blot detection of endogenous GAI3 protein in normal rat kidney (NRK) cell line. Ab14246 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-GNAI3 antibody (ab14246)Immunocytochemistry staining of endogenous GAI3 protein in AtT 20 (Left) and normal rat kidney (NRK) (right) cell lines. Ab14246 dilution: 1:200; staining using Alexa conjugated secondary antibody.
Datasheets and documents
References
ab14246 has not yet been referenced specifically in any publications.
