Anti-GNAI3 antibody (ab14246)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-GNAI3 antibody
See all GNAI3 primary antibodies -
Description
Chicken polyclonal to GNAI3 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Guinea pig, Human
Predicted to work with: Hamster
-
Immunogen
KNNLKECGLY
, corresponding to amino acids 345-354 of GAI3. -
Positive control
- Recombinant Human GNAI3 protein (ab124586) can be used as a positive control in WB. AtT 20 and Normal rat kidney (NRK) cell line.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab14246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). | |
| ICC/IF | 1/200. |
Target
-
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Cellular localization
Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
-
Database links
- Entrez Gene: 374097 Chicken
- Entrez Gene: 2773 Human
- Entrez Gene: 14679 Mouse
- Entrez Gene: 25643 Rat
- Omim: 139370 Human
- SwissProt: P38403 Guinea pig
- SwissProt: P08754 Human
- SwissProt: Q9DC51 Mouse
see all -
Alternative names
- 87U6 antibody
- FLJ26559 antibody
- G protein alpha inhibiting 3 antibody
see all
Images
-
Western blot - Anti-GNAI3 antibody (ab14246)Western blot detection of endogenous GAI3 protein in normal rat kidney (NRK) cell line using ab14246.
-
Immunocytochemistry/ Immunofluorescence - Anti-GNAI3 antibody (ab14246)Immunocytochemistry staining of endogenous GAI3 protein in AtT 20 (Left) and normal rat kidney (NRK) (right) cell lines. ab14246 dilution: 1/200; staining using Alexa conjugated secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Bhonker Y et al. The GPSM2/LGN GoLoco motifs are essential for hearing. Mamm Genome 27:29-46 (2016). Read more (PubMed: 26662512) »
