Overview

  • Product name

  • Description

    Chicken polyclonal to GNAI3
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Guinea pig, Human
    Predicted to work with: Hamster
  • Immunogen

    KNNLKECGLY

    , corresponding to amino acids 345-354 of GAI3.

  • Positive control

    • Recombinant Human GNAI3 protein (ab124586) can be used as a positive control in WB. AtT 20 and Normal rat kidney (NRK) cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab14246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF 1/200.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Cellular localization

    Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt
  • Database links

  • Alternative names

    • 87U6 antibody
    • FLJ26559 antibody
    • G protein alpha inhibiting 3 antibody
    • G(i) alpha 3 antibody
    • G(i) alpha-3 antibody
    • GNAI3 antibody
    • GNAI3_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody
    • Guanine nucleotide binding protein G(k) alpha subunit antibody
    • Guanine nucleotide-binding protein G(k) subunit alpha antibody
    • OTTHUMP00000013368 antibody
    see all

Images

  • Western blot detection of endogenous GAI3 protein in normal rat kidney (NRK) cell line using ab14246.

  • Immunocytochemistry staining of endogenous GAI3 protein in AtT 20 (Left) and normal rat kidney (NRK) (right) cell lines. ab14246 dilution: 1/200; staining using Alexa conjugated secondary antibody.

References

This product has been referenced in:

See 1 Publication for this product

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