Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAI3
  • Host species

    Rabbit
  • Specificity

    Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 68-354 of Human GNAI3 (Uniprot ID: P08754).

  • Positive control

    • 293T, H1299, HeLaS3, HepG2 cells; A431, MOLT4, Raji and Mouse brain whole cell lysates; Human SAS xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-GNAI3 antibody (ab154024) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : GNAI3 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 41 kDa

  • All lanes : Anti-GNAI3 antibody (ab154024) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 41 kDa



    7.5% SDS PAGE
  • Anti-GNAI3 antibody (ab154024) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.

References

This product has been referenced in:

  • Zhang Y  et al. Guanine and nucleotide binding protein 3 promotes odonto/osteogenic differentiation of apical papilla stem cells via JNK and ERK signaling pathways. Int J Mol Med 43:382-392 (2019). Read more (PubMed: 30431055) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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