Key features and details
- Rabbit polyclonal to GNAI3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GNAI3 antibody
See all GNAI3 primary antibodies
DescriptionRabbit polyclonal to GNAI3
Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 68-354 of Human GNAI3 (Uniprot ID: P08754).
- 293T, H1299, HeLaS3, HepG2 cells; A431, MOLT4, Raji and Mouse brain whole cell lysates; Human SAS xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
Cellular localizationCytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
- Information by UniProt
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All lanes : Anti-GNAI3 antibody (ab154024) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
7.5% SDS PAGE
Anti-GNAI3 antibody (ab154024) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 41 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.
ab154024 has been referenced in 1 publication.
- Zhang Y et al. Guanine and nucleotide binding protein 3 promotes odonto/osteogenic differentiation of apical papilla stem cells via JNK and ERK signaling pathways. Int J Mol Med 43:382-392 (2019). PubMed: 30431055